Product information "PureCube Compact Cartridge Co-NTA"
Type:
Cartridge
Bead size:
40 µm (Agarose)
Affinity :
His-tag
Purpose:
Protein Purification
Ligand:
NTA
Coupled Ion:
Co2+
FPLC based protein purification of His-tagged proteins is a common practice. Therefore we pre-filled our PureCube COMPACT Cartridges / Columns with our PureCube Co-NTA agarose resin. The columns are suited for most FPLC devices. However please check the corresponding "Features" table to confirm compability with your machinery. In case of no compability we offer cartridges / columns cartridges / columns of different formats pre-filled with Co-NTA. Co-NTA agarose COMPACT cartridges with 100 µm diameter agarose beads are to go-to option if higher flow rates are needed.
Features - Regarding Purification capabilities.
Feature
Usage
Specific binding and purification of 6x His-tagged proteins
Specifity
Affinity to His-tagged proteins
Binding capacity
>30 mg/mL
Bead Ligand
Co-NTA
Bead size
40 μm
Chelator stability
Stable in buffer containing 10 mM DTT and 1 mM EDTA
ph Stability
2-14
Features - Regarding Cartridge / Column itself.
Feature
PureCube Compact Cartridge 1 mL
PureCube Compact Cartridge 5 mL
Bed Volume
1 mL
5 mL
Max. Flow Rate
6 mL/min
Dimensions: diameter X length (mm)
5 X 35
17 X 35
Body material
Polypropylene
Inlet
10-32 UNF female thread
Outlet
10-32 UNF female thread
PDF Downloads
*The protocols on how to use the cartridges / columns are dependent on the used machine, therefore we unfortunately cannot provide a specific protocol here.
Related links to "PureCube Compact Cartridge Co-NTA"
