PureCube Co-NTA Agarose XL

PureCube Co-NTA Agarose XL
€140.00 *

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mL:

  • 55403
PureCube XL Co-NTA agarose consist of agarose beads with a diameter of around 400 µm. They... more
Product information "PureCube Co-NTA Agarose XL"
Type: Agarose
Bead size: 400 µm (XL Agarose)
Affinity : His-tag
Purpose: Protein Purification
Ligand: NTA
Coupled Ion: Co2+
PureCube XL Co-NTA agarose consist of agarose beads with a diameter of around 400 µm. They are used for extreme cases of His tag protein purifications. These extremes cases are defined by very low flow rates. XL beads can be used for FPLC assays to overcome this issue. For Co-NTA based His Tag purification we recommend our PureCube Co-NTA resin with 40 µm diameter and  our Purecube 100 Co-NTA agarose resin beads.

Features

Usage Specific binding and purification of 6x his-tagged proteins
Specifity Affinity to His-tagged proteins
Binding capacity >30 mg/mL
Chelator stability Stable in buffer containing 10 mM DTT and 1 mM EDTA
Filling quantity Delivered as a 50 % suspension
Bead size 400 μm
Bead Ligand Co-NTA
Required equipment
 
  • Lysis Buffer
  • Wash Buffer
  • Elution Buffer
  • Ice bath
  • Refrigerated centrifuge for 50 mL tube (min 10,000 x g)
  • 50 mL centrifuge tube
  • Micropipettor and Micropipetting tips
  • Disposable gravity flow columns with capped bottom outlet, 2 ml
  •  pH meter
  • End-over-end shaker
  • SDS-PAGE buffers, reagents and equipment Optional: Western Blot reagents and equipment
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