PureCube 100 Co-NTA Agarose

PureCube 100 Co-NTA Agarose
€118.00 *

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Delivery time appr. 1-3 workdays

ML:

  • 74403
Our PureCube 100 Co-NTA agarose consist of agarose resin beads with a diameter of 100 µm.... more
Product information "PureCube 100 Co-NTA Agarose"
Type: Agarose
Bead size: 100 µm (Agarose)
Affinity : His-tag
Purpose: Protein Purification
Ligand: NTA
Coupled Ion: Co2+
Our PureCube 100 Co-NTA agarose consist of agarose resin beads with a diameter of 100 µm. They are used for the purification of active & stable His Tag protein from e.g. cell lysates. Our agarose resins are suited for batch spin columns and FPLC. Co-NTA provides purer protein eluates that Ni-NTA. Furthermore we recommend our PureCube Co-NTA resin with 40 µm diameter and for cases that require a drastically increased flow rate of the beads our Purecube XL Co-NTA agarose beads.

Features

Usage Specific binding and purification of 6x his-tagged proteins
Specifity Affinity to His-tagged proteins
Binding capacity >30 mg/mL
Chelator stability Stable in buffer containing 10 mM DTT and 1 mM EDTA
Filling quantity Delivered as a 50 % suspension
Bead size 40 μm
Bead Ligand Co-NTA
Required equipment
 
  • Lysis Buffer
  • Wash Buffer
  • Elution Buffer
  • Ice bath
  • Refrigerated centrifuge for 50 mL tube (min 10,000 x g)
  • 50 mL centrifuge tube
  • Micropipettor and Micropipetting tips
  • Disposable gravity flow columns with capped bottom outlet, 2 ml
  •  pH meter
  • End-over-end shaker
  • SDS-PAGE buffers, reagents and equipment Optional: Western Blot reagents and equipment
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