Product information "PureCube 100 Co-NTA Cartridge"
Type:
Cartridge
Bead size:
100 µm
Since the time spend of filling cartridges / columns can be spend better, we offer our quality PureCube 100 Co-NTA agarose resin prefilled. The best option to get pure His Tagged Protein in a FPLC based method. The cartridges are compatible with most FPLC machinery like ÄKTAs. Refer to the corresponding features table to confirm compability with your hardware. In case of no compability with these columns listed here you should try out our 100 Co-NTA COMPACT Cartridges / Columns. For the magnetic separation process we offer our own modular PureCube MagBead separator for you.
Features - Regarding Purification capabilities.
Feature
Usage
Specific binding and purification of 6x his-tagged proteins
Specifity
Affinity to His-tagged proteins
Binding capacity
>30 mg/mL
Bead Ligand
Co-NTA
Bead size
100 μm
Matrix
6% highly cross-linked agarose
Chelator stability
Stable in buffer containing 10 mM DTT and 1 mM EDTA
ph Stability
2-14
Features - Regarding Cartridge / Column itself.
Feature
PureCube Compact Cartridge 1 mL
PureCube Compact Cartridge 5 mL
Bed Volume
1 mL
5 mL
Dimensions: diameter X length (mm)
6.2 x 50
11 x 80
Body material
Polypropylene
Acrylate
End Plug Material
Polypropylene
Inlet
10-32 UNF female thread
Outlet
10-32 UNF female thread
Max. Flow Rate
6 mL/min
PDF Downloads
*The protocols on how to use the cartridges / columns are dependent on the used machine, therefore we unfortunately cannot provide a specific protocol here.
