Product information "PureCube 100 Compact Cartridge Co-NTA"
Type:
Cartridge
Bead size:
100 µm (Agarose)
Affinity :
His-tag
Purpose:
Protein Purification
Ligand:
NTA
Coupled Ion:
Co2+
FPLC is one of the best ways to purify proteins. Therefore we pre-filled cartridges / columns with our high quality PureCube 100 Co-NTA agarose resin beads. Co-NTA based purification products are the best way to obtain maximum purity of his tagged protein combined with high yields. Please refer the features table to confirm if the format of these cartridges / colums match your FPLC machinery e.g. ÄKTA. If these columns do not match your hardware try out our other 100 Co-NTA Cartridges for matching compability.
Features - Regarding Purification capabilities.
Feature
Usage
Specific binding and purification of 6x his-tagged proteins
Specifity
Affinity to His-tagged proteins
Binding capacity
>30 mg/mL
Bead Ligand
Co-NTA
Bead size
100 μm
Matrix
6% highly cross-linked agarose
Chelator stability
Stable in buffer containing 10 mM DTT and 1 mM EDTA
ph Stability
2-14
Features - Regarding Cartridge / Column itself.
Feature
PureCube Compact Cartridge 1 mL
PureCube Compact Cartridge 5 mL
Bed Volume
1 mL
5 mL
Dimensions: diameter X length (mm)
8.0 x 35
17 x 35
Body material
Polypropylene
End Plug Material
Polypropylene
Inlet
10-32 UNF female thread
Outlet
10-32 UNF female thread
Max. Flow Rate
6 mL/min
PDF Downloads
*The protocols on how to use the cartridges / columns are dependent on the used machine, therefore we unfortunately cannot provide a specific protocol here.
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