PureCube 100 INDIGO Ni-Cartridge

€57.00 *

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mL:

  • 75301
The PureCube INDIGO-Ni Column / Cartridge is an IMAC chromatography column prepacked with... more
Product information "PureCube 100 INDIGO Ni-Cartridge"
Type: Cartridge
Bead size: 100 µm (Agarose)
Affinity : His-tag
Purpose: Protein Purification
Ligand: INDIGO
Coupled Ion: Ni2+


The PureCube INDIGO-Ni Column / Cartridge is an IMAC chromatography column prepacked with PureCube 100 INDIGO-Ni Ni-IDA Agarose resin beads. You can choose between two sizes, 1 mL bed volume and 5 mL bed volume. Please have a look at the given features table to ensure if the Cartridge / Column is compatible with your FPLC hardware. If there are lacking compatibility we recommed to check out our INDIGO-Ni COMPACT Cartridges , that are covering additional compabilities.

Features - Regarding Purification capabilities.

Feature  
Usage Specific binding and purification of 6x his-tagged proteins
Specifity Affinity to His-tagged proteins
Binding capacity >100 mg/mL
Bead Ligand INDIGO-Ni
Bead size 100 μm
Matrix 6% highly cross-linked agarose
Chelator stability Stable in buffer containing 20 mM DTT and 20 mM EDTA
ph Stability 2-13

Features - Regarding Cartridge / Column itself.

Feature PureCube Cartridge 1 mL PureCube Cartridge 5 mL
Bed Volume 1 mL 5 mL
Dimensions: diameter X length (mm) 6.2 x 50 11 x 80
Body material Polypropylene Acrylate
End Plug Material Polypropylene
Inlet 10-32 UNF female thread
Outlet 10-32 UNF female thread
Max. Flow Rate 6 mL/min

PDF Downloads

 
 
*The protocols on how to use the cartridges / columns are dependent on the used machine, therefore we unfortunately cannot provide a specific protocol here.
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