Product information "PureCube 100 Compact Cartridge Ni-INDIGO"
Type:
Cartridge
Bead size:
100 µm (Agarose)
Affinity :
His-tag
Purpose:
Protein Purification
Ligand:
INDIGO
Coupled Ion:
Ni2+
The PureCube INDIGO-Ni COMPACT Column / Cartridge provides you with our high quality PureCube 100 INDIGO-Ni agarose resin pre-packed into an an IMAC chromatography column. We offer two different sizes, 1 mL bed volume and 5 mL bed volume. Please have a look at the given compability features table to ensure if the Cartridge / Column fits with your FPLC hardware. If there are lacking compatibility we recommed to check out our other PureCube INDIGO-Ni Cartridges / Columns, that are covering additional compabilities.
Features - Regarding Purification capabilities.
Feature
Usage
Specific binding and purification of 6x his-tagged proteins
Specifity
Affinity to His-tagged proteins
Binding capacity
>100 mg/mL
Bead Ligand
INDIGO-Ni
Bead size
100 μm
Chelator stability
Stable in buffer containing 20 mM DTT and 20 mM EDTA
ph Stability
2-13
Features - Regarding Cartridge / Column itself.
Feature
PureCube Compact Cartridge 1 mL
PureCube Compact Cartridge 5 mL
Bed Volume
1 mL
5 mL
Max. Flow Rate
6 mL/min
Dimensions: diameter X length (mm)
5 X 35
17 X 35
Body material
Polypropylene
Inlet
10-32 UNF female thread
Outlet
10-32 UNF female thread
PDF Downloads
*The protocols on how to use the cartridges / columns are dependent on the used machine, therefore we unfortunately cannot provide a specific protocol here.
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