PureCube Ni-IDA Agarose

PureCube Ni-IDA Agarose
€83.00 *

Prices plus VAT plus shipping costs

Ready to ship today,
Delivery time appr. 1-3 workdays

mL:

  • 30103
PureCube Ni-IDA agarose resins for His-tag protein purification are small agarose beads (Ø... more
Product information "PureCube Ni-IDA Agarose"
Type: Agarose
Bead size: 40 µm (Agarose)
Affinity : His-tag
Purpose: Protein Purification
Ligand: IDA
Coupled Ion: Ni2+
PureCube Ni-IDA agarose resins for His-tag protein purification are small agarose beads (Ø 40 µm) for batch spin columns and FPLC. Their small size provides them with great mechanical stability. Their great protein yield of at least 70 mg of pure His-tagged per ml resin also comes from the high density of Ni-IDA groups in the suspension due to the small bead size and subsequent huge total surface area of the beads.

Features

Usage Specific binding and purification of 6x His-tagged proteins
Specifity Affinity to His-tagged proteins
Binding capacity >70 mg/mL
Bead Ligand Ni-IDA
Bead size 40 μm
Chelator stability Stable in buffer containing 10 mM DTT and 1 mM EDTA
Filling quantity Delivered as a 50 % suspension
Required equipment
 
  • Lysis Buffer
  • Wash Buffer
  • Elution Buffer
  • Ice bath
  • Refrigerated centrifuge for 50 mL tube (min 10,000 x g)
  • 50 mL centrifuge tube
  • Micropipettor and Micropipetting tips
  • Disposable gravity flow columns with capped bottom outlet, 2 ml
  • pH meter
  • End-over-end shaker
  • SDS-PAGE buffers, reagents and equipment Optional: Western Blot reagents and equipment
Related links to "PureCube Ni-IDA Agarose"
PureCube HiCap StrepTactin MagBeads PureCube HiCap StrepTactin MagBeads
Article number: 34205
Sales price: From €92.00 *
PureCube HiCap StrepTactin Agarose PureCube HiCap StrepTactin Agarose
Article number: 34103
Sales price: From €83.00 *
Rho Starter Set 1: PureCube Rho1D4 Agarose (1 ml) + Rho1D4 peptide (5 mg) Rho Starter Set 1: PureCube Rho1D4 Agarose (1...
Article number: 33199
Sales price: €197.00 * €394.00 *
PureCube Ni-NTA Agarose PureCube Ni-NTA Agarose
Article number: 31103
Sales price: From €119.00 *
PureCube Ni-IDA Cartridge PureCube Ni-IDA Cartridge
Article number: 30301
Sales price: From €39.00 *
PureCube Ni-IDA MagBeads PureCube Ni-IDA MagBeads
Article number: 30201
Sales price: From €51.00 *
PureCube Ni-NTA Agarose PureCube Ni-NTA Agarose
Article number: 31103
Sales price: From €119.00 *
PureCube Ni-IDA MagBeads PureCube Ni-IDA MagBeads
Article number: 30201
Sales price: From €51.00 *
PureCube 100 INDIGO Ni-Agarose PureCube 100 INDIGO Ni-Agarose
Article number: 75103
Sales price: From €126.00 *
PureCube Co-NTA Cartridge PureCube Co-NTA Cartridge
Article number: 31601
Sales price: From €44.00 *
PureCube Ni-NTA MagBeads PureCube Ni-NTA MagBeads
Article number: 31201
Sales price: From €74.00 *
PureCube 100 Ni-NTA Agarose PureCube 100 Ni-NTA Agarose
Article number: 74103
Sales price: From €119.00 *
PureCube Ni-NTA MagBeads XL PureCube Ni-NTA MagBeads XL
Article number: 55305
Sales price: From €402.00 *
PureCube Epoxy Activated Agarose PureCube Epoxy Activated Agarose
Article number: 50703
Sales price: From €123.00 *
PureCube Compact Cartridge Ni-IDA PureCube Compact Cartridge Ni-IDA
Article number: 30302
Sales price: From €39.00 *
PureCube Ni-IDA Cartridge PureCube Ni-IDA Cartridge
Article number: 30301
Sales price: From €39.00 *
PureCube Compact Cartridge Al-NTA PureCube Compact Cartridge Al-NTA
Article number: 31602-Al
Sales price: From €44.00 *
PureCube Fe-NTA Cartridge PureCube Fe-NTA Cartridge
Article number: 31601-Fe
Sales price: From €44.00 *
PureCube INDIGO Ni-MagBeads PureCube INDIGO Ni-MagBeads
Article number: 75201
Sales price: From €81.00 *
PureCube Ni-NTA Agarose XL PureCube Ni-NTA Agarose XL
Article number: 55103
Sales price: From €158.00 *
PureCube IDA Agarose PureCube IDA Agarose
Article number: 30703
Sales price: From €83.00 *
SMALP Screening Set (4x3x50 mg) SMALP Screening Set (4x3x50 mg)
Article number: 18280
Sales price: €291.00 *
PureCube Ti-NTA MagBeads PureCube Ti-NTA MagBeads
Article number: 31501-Ti
Sales price: From €80.00 *
PureCube Fe-NTA MagBeads PureCube Fe-NTA MagBeads
Article number: 31501-Fe
Sales price: From €80.00 *
PureCube 100 Compact Cartridge Co-NTA PureCube 100 Compact Cartridge Co-NTA
Article number: 74602
Sales price: From €59.00 *
PureCube 100 Co-NTA Agarose PureCube 100 Co-NTA Agarose
Article number: 74403
Sales price: From €126.00 *
PureCube Co-NTA Agarose XL PureCube Co-NTA Agarose XL
Article number: 55403
Sales price: From €158.00 *
PureCube Maleimide Activated MagBeads PureCube Maleimide Activated MagBeads
Article number: 51201
Sales price: From €265.00 *
Rho Starter Set 3: PureCube Rho1D4 Cartridge (1x1 ml) + Rho1D4 peptide (5 mg) Rho Starter Set 3: PureCube Rho1D4 Cartridge...
Article number: 33399
Sales price: €231.00 * €462.00 *
AASTY 11-50 AASTY 11-50
Article number: 18265
Sales price: From €209.00 *
Overview of all Synthetic polymers that are contained in the MAXI screening Kit Synthetic Nanodisc Screening Kit MAXI (16x2x50 mg)
Article number: 18295
Sales price: €650.00 *
AASTY Screening Kit Contents AASTY Screening Kit (6x2x50 mg)
Article number: 18285
Sales price: €231.00 *
SMALP 200 SMALP 200
Article number: 18210
Sales price: From €209.00 *
HisCube - Ni-INDIGO His-Tag Protein Purification MINI Kit HisCube - Ni-INDIGO His-Tag Protein...
Article number: 80101
Sales price: €534.00 *
Vial containing DIBMA 12 HEPES DIBMA 12, HEPES
Article number: 18011
Sales price: From €209.00 *
PureCube Zn-NTA Cartridge PureCube Zn-NTA Cartridge
Article number: 31601-Zn
Sales price: From €44.00 *
PureCube Cu-NTA Agarose PureCube Cu-NTA Agarose
Article number: 31403-Cu
Sales price: From €125.00 *
PureCube Al-NTA Agarose PureCube Al-NTA Agarose
Article number: 31403-Al
Sales price: From €125.00 *
PureCube 100 NTA Agarose PureCube 100 NTA Agarose
Article number: 74703
Sales price: From €126.00 *
PureCube 100 Ni-NTA Cartridge PureCube 100 Ni-NTA Cartridge
Article number: 74301
Sales price: From €57.00 *
L-Glutathione reduced L-Glutathione reduced
Article number: 61035
Sales price: €130.00 *
PureCube Ni-IDA MagBeads XL PureCube Ni-IDA MagBeads XL
Article number: 55205
Sales price: From €258.00 *
PureCube Ni-IDA Agarose XL PureCube Ni-IDA Agarose XL
Article number: 55003
Sales price: From €119.00 *
PureCube Carboxy MagBeads PureCube Carboxy MagBeads
Article number: 50201
Sales price: From €99.00 *
PureCube HiCap StrepTactin Agarose PureCube HiCap StrepTactin Agarose
Article number: 34103
Sales price: From €83.00 *
PureCube Rho1D4 Cartridge PureCube Rho1D4 Cartridge
Article number: 33301
Sales price: From €338.00 *
Viewed