PureCube Ni-IDA Agarose

PureCube Ni-IDA Agarose
€73.00 *

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  • 30103
PureCube Ni-IDA agarose resins for His-tag protein purification are small agarose beads (Ø... more
Product information "PureCube Ni-IDA Agarose"
Type: Agarose
Bead size: 40 µm (Agarose)
Affinity : His-tag
Purpose: Protein Purification
Ligand: IDA
Coupled Ion: Ni2+
PureCube Ni-IDA agarose resins for His-tag protein purification are small agarose beads (Ø 40 µm) for batch spin columns and FPLC. Their small size provides them with great mechanical stability. Their great protein yield of at least 70 mg of pure His-tagged per ml resin also comes from the high density of Ni-IDA groups in the suspension due to the small bead size and subsequent huge total surface area of the beads.

Features

Usage Specific binding and purification of 6x His-tagged proteins
Specifity Affinity to His-tagged proteins
Binding capacity >70 mg/mL
Bead Ligand Ni-IDA
Bead size 40 μm
Chelator stability Stable in buffer containing 10 mM DTT and 1 mM EDTA
Filling quantity Delivered as a 50 % suspension
Required equipment
 
  • Lysis Buffer
  • Wash Buffer
  • Elution Buffer
  • Ice bath
  • Refrigerated centrifuge for 50 mL tube (min 10,000 x g)
  • 50 mL centrifuge tube
  • Micropipettor and Micropipetting tips
  • Disposable gravity flow columns with capped bottom outlet, 2 ml
  • pH meter
  • End-over-end shaker
  • SDS-PAGE buffers, reagents and equipment Optional: Western Blot reagents and equipment
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