PureCube Ni-IDA Agarose

PureCube Ni-IDA Agarose
€73.00 *

Prices plus VAT plus shipping costs

Ready to ship today,
Delivery time appr. 1-3 workdays

ML:

  • 30103
PureCube Ni-IDA agarose resins for His-tag protein purification are small agarose beads (Ø... more
Product information "PureCube Ni-IDA Agarose"
Type: Agarose
Bead size: 40 µm (Agarose)
Affinity : His-tag
Purpose: Protein Purification
Ligand: IDA
Coupled Ion: Ni2+
PureCube Ni-IDA agarose resins for His-tag protein purification are small agarose beads (Ø 40 µm) for batch spin columns and FPLC. Their small size provides them with great mechanical stability. Their great protein yield of at least 70 mg of pure His-tagged per ml resin also comes from the high density of Ni-IDA groups in the suspension due to the small bead size and subsequent huge total surface area of the beads.

Features

Usage Specific binding and purification of 6x His-tagged proteins
Specifity Affinity to His-tagged proteins
Binding capacity >70 mg/mL
Bead Ligand Ni-IDA
Bead size 40 μm
Chelator stability Stable in buffer containing 10 mM DTT and 1 mM EDTA
Filling quantity Delivered as a 50 % suspension
Required equipment
 
  • Lysis Buffer
  • Wash Buffer
  • Elution Buffer
  • Ice bath
  • Refrigerated centrifuge for 50 mL tube (min 10,000 x g)
  • 50 mL centrifuge tube
  • Micropipettor and Micropipetting tips
  • Disposable gravity flow columns with capped bottom outlet, 2 ml
  • pH meter
  • End-over-end shaker
  • SDS-PAGE buffers, reagents and equipment Optional: Western Blot reagents and equipment
Related links to "PureCube Ni-IDA Agarose"
PureCube HiCap StrepTactin MagBeads PureCube HiCap StrepTactin MagBeads
Article number: 34201
Sales price: From €81.00 *
PureCube HiCap StrepTactin Agarose PureCube HiCap StrepTactin Agarose
Article number: 34101
Sales price: From €73.00 *
Rho Starter Set 1: PureCube Rho1D4 Agarose (1 ml) + Rho1D4 peptide (5 mg) Rho Starter Set 1: PureCube Rho1D4 Agarose (1...
Article number: 33199
Sales price: €175.00 * €350.00 *
PureCube Ni-NTA Agarose PureCube Ni-NTA Agarose
Article number: 31103
Sales price: From €103.00 *
PureCube 100 INDIGO Ni-Agarose PureCube 100 INDIGO Ni-Agarose
Article number: 75103
Sales price: From €112.00 *
PureCube Co-NTA Agarose PureCube Co-NTA Agarose
Article number: 31403
Sales price: From €111.00 *
PureCube 100 Compact Cartridge Ni-INDIGO PureCube 100 Compact Cartridge Ni-INDIGO
Article number: 75302
Sales price: From €52.00 *
PureCube Ni-NTA Agarose XL PureCube Ni-NTA Agarose XL
Article number: 55103
Sales price: From €140.00 *
PureCube Glutathione Agarose PureCube Glutathione Agarose
Article number: 32103
Sales price: From €145.00 *
PureCube Compact Cartridge PureCube Compact Cartridge
Article number: 16912
Sales price: From €24.00 *
PureCube 100 Co-NTA Agarose PureCube 100 Co-NTA Agarose
Article number: 74403
Sales price: From €118.00 *
PureCube Ni-IDA Agarose XL PureCube Ni-IDA Agarose XL
Article number: 55003
Sales price: From €105.00 *
PureCube Maleimide Activated MagBeads PureCube Maleimide Activated MagBeads
Article number: 51201
Sales price: From €235.00 *
PureCube Compact Cartridge Glutathione PureCube Compact Cartridge Glutathione
Article number: 32302
Sales price: From €42.00 *
PureCube Co-NTA Cartridge PureCube Co-NTA Cartridge
Article number: 31601
Sales price: From €39.00 *
PureCube Co-NTA MagBeads PureCube Co-NTA MagBeads
Article number: 31501
Sales price: From €71.00 *
PureCube Ni-NTA Cartridge PureCube Ni-NTA Cartridge
Article number: 31301
Sales price: From €36.00 *
GST-eGFP fusion protein, DYKDDDDK-tag GST-eGFP fusion protein, DYKDDDDK-tag
Article number: 29921
Sales price: From €226.00 *
Nanodisc MSP1D1-His POPC (50 µL) Nanodisc MSP1D1-His POPC (50 µL)
Article number: 26313
Sales price: €424.00 *
MSP1D1-His, lyophilized protein MSP1D1-His, lyophilized protein
Article number: 26112
Sales price: From €131.00 *
PureCube Al-NTA MagBeads PureCube Al-NTA MagBeads
Article number: 31501-Al
Sales price: From €71.00 *
PureCube 100 INDIGO Ni-Cartridge PureCube 100 INDIGO Ni-Cartridge
Article number: 75301
Sales price: From €50.00 *
PureCube INDIGO Ni-MagBeads PureCube INDIGO Ni-MagBeads
Article number: 75201
Sales price: From €72.00 *
PureCube 1-step batch Midi Plus PureCube 1-step batch Midi Plus
Article number: 63203
Sales price: From €49.00 *
PureCube Maleimide Activated Agarose PureCube Maleimide Activated Agarose
Article number: 51103
Sales price: From €311.00 *
PureCube Amine Activated Agarose PureCube Amine Activated Agarose
Article number: 51003
Sales price: From €106.00 *
PureCube Rho1D4 Agarose PureCube Rho1D4 Agarose
Article number: 33101
Sales price: From €239.00 *
PureCube Compact Cartridge Co-NTA PureCube Compact Cartridge Co-NTA
Article number: 31602
Sales price: From €39.00 *
PureCube Compact Cartridge Ni-NTA PureCube Compact Cartridge Ni-NTA
Article number: 31302
Sales price: From €36.00 *
PureCube Ni-NTA MagBeads PureCube Ni-NTA MagBeads
Article number: 31201
Sales price: From €65.00 *
PureCube Ni-IDA MagBeads PureCube Ni-IDA MagBeads
Article number: 30201
Sales price: From €45.00 *
GLP1R protein GLP1R protein
Article number: 28201
Sales price: From €1,071.00 *
Nanodisc MSP2N2-His POPC (50 µL) Nanodisc MSP2N2-His POPC (50 µL)
Article number: 26373
Sales price: €519.00 *
Nanodisc MSP1E3D1-His DMPC (50 µL) Nanodisc MSP1E3D1-His DMPC (50 µL)
Article number: 26351
Sales price: €424.00 *
Nanodisc Assembly Kit MSP1D1-His_DMPC Nanodisc Assembly Kit MSP1D1-His_DMPC
Article number: 26211
Sales price: €253.00 *
HisCube - Ni-INDIGO His-Tag Protein Purification MINI Kit HisCube - Ni-INDIGO His-Tag Protein...
Article number: 80101
Sales price: €474.00 *
PureCube Ti-NTA MagBeads PureCube Ti-NTA MagBeads
Article number: 31501-Ti
Sales price: From €71.00 *
DIBMA 10, HEPES DIBMA 10, HEPES
Article number: 18001
Sales price: From €185.00 *
PureCube Compact Cartridge Zn-NTA PureCube Compact Cartridge Zn-NTA
Article number: 31602-Zn
Sales price: From €39.00 *
PureCube Compact Cartridge Fe-NTA PureCube Compact Cartridge Fe-NTA
Article number: 31602-Fe
Sales price: From €39.00 *
PureCube Compact Cartridge Cu-NTA PureCube Compact Cartridge Cu-NTA
Article number: 31602-Cu
Sales price: From €39.00 *
PureCube Zn-NTA MagBeads PureCube Zn-NTA MagBeads
Article number: 31501-Zn
Sales price: From €71.00 *
PureCube Fe-NTA MagBeads PureCube Fe-NTA MagBeads
Article number: 31501-Fe
Sales price: From €71.00 *
Viewed