PureCube Ni-IDA MagBeads were developed for the affinity purification of proteins carrying a polyhistidine (His-) tag. The affinity matrix is based on spherical magnetic agarose beads, consisting of 6% cross agarose. Alternatively we offer Ni-IDA MagBeads also XL sized (90 µm bead diameter). For the magnetic separation we provide our own PureCube MagBead separator
Features - Regarding Purification capabilities.
Feature
Usage
Specific binding and purification of 6x his-tagged proteins
Specifity
Affinity to His-tagged proteins
Binding capacity
>40 mg/mL
Bead Ligand
Ni-IDA
Bead size
30 µm
Filling quantity
Delivered as a 25 % suspension
Chelator stability
Stable in buffer containing 10 mM DTT and 1 mM EDTA
pH stability
2-14
Other stabilities
100% methanol, 100% ethanol, 8 M urea, 6 M guanidinium hydrochloride, 30% (v/v) acetonitrile
