Product information "PureCube Compact Cartridge Ni-IDA"
Type:
Cartridge
Bead size:
40 µm (Agarose)
Affinity :
His-tag
Purpose:
Protein Purification
Ligand:
IDA
Coupled Ion:
Ni2+
Our PurceCube COMPACT Cartridge pre-filled with Ni-IDA is a chromatography column for the His-tag purification of proteins. It is compatibile with most FPLC machinery e.g. ÄKTAs. Please check the corresponding features table to ensure said compability with your given hardware. In case there is no compability to our COMPACT cartridges we advice you to try out our other pre-filled Ni-IDA Cartridges.
Features - Regarding Purification capabilities.
Feature
Usage
Specific binding and purification of 6x His-tagged proteins
Specifity
Affinity to His-tagged proteins
Binding capacity
>50 mg/mL
Bead Ligand
Ni-IDA
Bead size
40 μm
Chelator stability
Stable in buffer containing 10 mM DTT and 1 mM EDTA
ph Stability
2-14
Features - Regarding Cartridge / Column itself.
Feature
PureCube Compact Cartridge 1 mL
PureCube Compact Cartridge 5 mL
Bed Volume
1 mL
5 mL
Max. Flow Rate
6 mL/min
Dimensions: diameter X length (mm)
5 X 35
17 X 35
Body material
Polypropylene
Inlet
10-32 UNF female thread
Outlet
10-32 UNF female thread
PDF Downloads
*The protocols on how to use the cartridges / columns are dependent on the used machine, therefore we unfortunately cannot provide a specific protocol here.
Related links to "PureCube Compact Cartridge Ni-IDA"
