PureCube Ni-NTA Agarose

PureCube Ni-NTA Agarose
€103.00 *

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ML:

  • 31103
Our PureCube Ni-NTA agarose resins are small beads with a diameter of 40 µm. They are used... more
Product information "PureCube Ni-NTA Agarose"
Type: Agarose
Bead size: 40 µm (Agarose)
Affinity : His-tag
Purpose: Protein Purification
Ligand: NTA
Coupled Ion: Ni2+
Our PureCube Ni-NTA agarose resins are small beads with a diameter of 40 µm. They are used for the purification of active His tagged proteins from cells. Our year long experience in manufacturing agarose resin lead to the high yield of 80 mg protein per ml resin, which is leading in the market compared to other Ni-NTA suppliers. PureCube Ni-NTA resins are suited for batch spin columns and FPLC. Their small diameter provides them with a great mechanical stability. For higher flow rates we recommend our Ni-NTA agarose beads with 100 µm diameter and for extreme cases our XL sized Ni-NTA beads.

Features

Usage Specific binding and purification of 6x His tagged proteins
Specifity Affinity to His tagged proteins
Binding capacity >80 mg/mL
Bead Ligand Ni-NTA
Bead size 40 μm
Chelator stability Stable in buffer containing 10 mM DTT and 1 mM EDTA
Filling quantity Delivered as a 50 % suspension
Required equipment
 
  • Lysis Buffer
  • Wash Buffer
  • Elution Buffer
  • Ice bath
  • Refrigerated centrifuge for 50 mL tube (min 10,000 x g)
  • 50 mL centrifuge tube
  • Micropipettor and Micropipetting tips
  • Disposable gravity flow columns with capped bottom outlet, 2 ml
  •  pH meter
  • End-over-end shaker
  • SDS-PAGE buffers, reagents and equipment Optional: Western Blot reagents and equipment
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