PureCube Ni-NTA Agarose XL

€140.00 *

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Order number: 55103

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Our PureCube XL Ni-NTA agarose resins are beads with an average diameter of ~400 µm. They... more

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Product information "PureCube Ni-NTA Agarose XL"

Type:	Agarose
Bead size:	400 µm (XL Agarose)
Affinity :	His-tag
Purpose:	Protein Purification
Ligand:	NTA
Coupled Ion:	Ni2+

Our PureCube XL Ni-NTA agarose resins are beads with an average diameter of ~400 µm. They are used for the purification of His tagged proteins from cells. 400 µm agarose beads are a special creation by us for assays in which the flow rate is compromised by smaller beads. For higher protein yield we recommend our PureCube Ni-NTA agarose beads with a 40 µm diameter and our PureCube 100 Ni-NTA resin beads with a 100µm diameter.

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Features

Usage	Specific binding and purification of 6x his-tagged proteins
Specifity	Affinity to His-tagged proteins
Bead size	~400 μm
Binding capacity	>20 mg/mL
Bead Ligand	Ni-NTA
Chelator stability	Stable in buffer containing 10 mM DTT and 1 mM EDTA
Filling quantity	Delivered as a 50 % suspension
Required equipment	Lysis Buffer Wash Buffer Elution Buffer Ice bath Refrigerated centrifuge for 50 mL tube (min 10,000 x g) 50 mL centrifuge tube Micropipettor and Micropipetting tips Disposable gravity flow columns with capped bottom outlet, 2 ml  pH meter End-over-end shaker SDS-PAGE buffers, reagents and equipment Optional: Western Blot reagents and equipment

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