PureCube Ni-NTA Agarose XL

PureCube Ni-NTA Agarose XL
€158.00 *

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Delivery time appr. 1-3 workdays

mL:

  • 55103
Our PureCube XL Ni-NTA agarose resins are beads with an average diameter of ~400 µm. They... more
Product information "PureCube Ni-NTA Agarose XL"
Type: Agarose
Bead size: 400 µm (XL Agarose)
Affinity : His-tag
Purpose: Protein Purification
Ligand: NTA
Coupled Ion: Ni2+
Our PureCube XL Ni-NTA agarose resins are beads with an average diameter of ~400 µm. They are used for the purification of His tagged proteins from cells. 400 µm agarose beads are a special creation by us for assays in which the flow rate is compromised by smaller beads. For higher protein yield we recommend our PureCube Ni-NTA agarose beads with a 40 µm diameter and our PureCube 100 Ni-NTA resin beads with a 100µm diameter.

Features

Usage Specific binding and purification of 6x his-tagged proteins
Specifity Affinity to His-tagged proteins
Bead size ~400 μm
Binding capacity >20 mg/mL
Bead Ligand Ni-NTA
Chelator stability Stable in buffer containing 10 mM DTT and 1 mM EDTA
Filling quantity Delivered as a 50 % suspension
Required equipment
 
  • Lysis Buffer
  • Wash Buffer
  • Elution Buffer
  • Ice bath
  • Refrigerated centrifuge for 50 mL tube (min 10,000 x g)
  • 50 mL centrifuge tube
  • Micropipettor and Micropipetting tips
  • Disposable gravity flow columns with capped bottom outlet, 2 ml
  •  pH meter
  • End-over-end shaker
  • SDS-PAGE buffers, reagents and equipment Optional: Western Blot reagents and equipment
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