Our PureCube 100 Ni-NTA agarose resins are beads with a diameter of 100 µm. They are used for the purification of active His tagged proteins from cells. Years of experience in manufacturing and developing agarose beads have accumulated to the high protein yield of 80 mg protein per ml resin, which is leading in the market compared to other Ni-NTA suppliers. PureCube 100 Ni-NTA resins are suited for FPLC and batch spin columns. For more mechanical stable agarose beads we recommend our Ni-NTA agarose beads with 40 µm diameter and if an even higher flow rate is desired our XL sized Ni-NTA beads.
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Features
Usage
Specific binding and purification of 6x his-tagged proteins
Specifity
Affinity to His-tagged proteins
Bead size
100 μm
Binding capacity
>80 mg/mL
Bead Ligand
Ni-NTA
Chelator stability
Stable in buffer containing 10 mM DTT and 1 mM EDTA
Filling quantity
Delivered as a 50 % suspension
Required equipment
Lysis Buffer
Wash Buffer
Elution Buffer
Ice bath
Refrigerated centrifuge for 50 mL tube (min 10,000 x g)
50 mL centrifuge tube
Micropipettor and Micropipetting tips
Disposable gravity flow columns with capped bottom outlet, 2 ml
pH meter
End-over-end shaker
SDS-PAGE buffers, reagents and equipment Optional: Western Blot reagents and equipment
