PureCube 100 Ni-NTA Cartridge

€50.00 *

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  • 74301
We offer our 100 PureCube Ni-NTA agarose resin pre-filled in Columns / Cartridges for FPLC... more
Product information "PureCube 100 Ni-NTA Cartridge"
Type: Cartridge
Bead size: 100 µm


We offer our 100 PureCube Ni-NTA agarose resin pre-filled in Columns / Cartridges for FPLC based His Tag protein purification. The columns / cartridges are compatible with many FPLC machines like ÄKTAs. Consult the corresponding features table to find out if they are matching your hardware. Alternatively we also offer columns / cartridged with different measurements that we call COMPACT cartridges / columns in case these cartridges here are not compatible with your hardware. Alternatively we offer the same cartridges filled with smaller Ni-NTA beads (40 µm) if protein yield is of more importance than flow rates.

Features - Regarding Purification capabilities.

Feature  
Usage Specific binding and purification of 6x his-tagged proteins
Specifity Affinity to His-tagged proteins
Binding capacity >80 mg/mL
Bead Ligand Ni-NTA
Bead size 100 μm
Matrix 6% highly cross-linked agarose
Chelator stability Stable in buffer containing 10 mM DTT and 1 mM EDTA
ph Stability 2-14

Features - Regarding Cartridge / Column itself.

Feature PureCube Compact Cartridge 1 mL PureCube Compact Cartridge 5 mL
Bed Volume 1 mL 5 mL
Dimensions: diameter X length (mm) 6.2 x 50 11 x 80
Body material Polypropylene Acrylate
End Plug Material Polypropylene
Inlet 10-32 UNF female thread
Outlet 10-32 UNF female thread
Max. Flow Rate 6 mL/min

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*The protocols on how to use the cartridges / columns are dependent on the used machine, therefore we unfortunately cannot provide a specific protocol here.
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