We offer our 100 PureCube Ni-NTA agarose resin pre-filled in Columns / Cartridges for FPLC... more
Product information "PureCube 100 Ni-NTA Cartridge"
|Bead size:||100 µm (Agarose)|
We offer our 100 PureCube Ni-NTA agarose resin pre-filled in Columns / Cartridges for FPLC based His Tag protein purification. The columns / cartridges are compatible with many FPLC machines like ÄKTAs. Consult the corresponding features table to find out if they are matching your hardware. Alternatively we also offer columns / cartridged with different measurements that we call COMPACT cartridges / columns in case these cartridges here are not compatible with your hardware. Alternatively we offer the same cartridges filled with smaller Ni-NTA beads (40 µm) if protein yield is of more importance than flow rates.
Features - Regarding Purification capabilities.
|Usage||Specific binding and purification of 6x his-tagged proteins|
|Specifity||Affinity to His-tagged proteins|
|Binding capacity||>80 mg/mL|
|Bead size||100 μm|
|Matrix||6% highly cross-linked agarose|
|Chelator stability||Stable in buffer containing 10 mM DTT and 1 mM EDTA|
Features - Regarding Cartridge / Column itself.
|Feature||PureCube Compact Cartridge 1 mL||PureCube Compact Cartridge 5 mL|
|Bed Volume||1 mL||5 mL|
|Dimensions: diameter X length (mm)||6.2 x 50||11 x 80|
|End Plug Material||Polypropylene|
|Inlet||10-32 UNF female thread|
|Outlet||10-32 UNF female thread|
|Max. Flow Rate||6 mL/min|
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