Product information "PureCube 100 Compact Cartridge Ni-NTA"
Type:
Cartridge
Bead size:
100 µm (Agarose)
Affinity :
His-tag
Purpose:
Protein Purification
Ligand:
NTA
Coupled Ion:
Ni2+
We offer our 100 PureCube Ni-NTA Aaarose resin pre-filled in COMPACT Columns / Cartridges for FPLC based His Tag purification. The COMPACT columns / cartridges are compatible with many FPLC machines like ÄKTA. Compare your hardware with the features listed on the corresponding table to see if the COMPACT cartridges are matching your setup. In case columns with different measurements are needed we offer these cartridges / columns filled with PureCube 100 Ni-NTA resin. Alternatively we offer our same COMPACT cartridges filled with smaller Ni-NTA beads (40 µm) if protein yield is of more importance than flow rates.
Features - Regarding Purification capabilities.
Feature
Usage
Specific binding and purification of 6x his-tagged proteins
Specifity
Affinity to His-tagged proteins
Binding capacity
>80 mg/mL
Bead Ligand
Ni-NTA
Bead size
100 μm
Matrix
6% highly cross-linked agarose
Chelator stability
Stable in buffer containing 10 mM DTT and 1 mM EDTA
ph Stability
2-14
Features - Regarding Cartridge / Column itself.
Feature
PureCube Compact Cartridge 1 mL
PureCube Compact Cartridge 5 mL
Bed Volume
1 mL
5 mL
Dimensions: diameter X length (mm)
8.0 x 35
17 x 35
Body material
Polypropylene
End Plug Material
Polypropylene
Inlet
10-32 UNF female thread
Outlet
10-32 UNF female thread
Max. Flow Rate
6 mL/min
PDF Downloads
*The protocols on how to use the cartridges / columns are dependent on the used machine, therefore we unfortunately cannot provide a specific protocol here.
Related links to "PureCube 100 Compact Cartridge Ni-NTA"
