PureCube Butyl Agarose

Order number: 39003

€76.00*

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Description

Hydrophobic interaction chromatography (HIC) is a reliable method to separate proteins and other biomolecules based on their hydrophobicity. Our PureCube Butyl agarose resin is a suspension of purification beads for such an approach. If you use HIC with your sample for the first time we recommend testing all of our HIC resins before.
These include:
Feature
Usage As a matriy for Hydrophobic Interaction Chromatography (HIC)
Specificity Hydrophobic biomolecules and proteins
Bead size 40 μm
Bead Ligand Butyl groups
Butyl group density 40 µmol/ml
BSA binding capacity 25 mg/ml
lysozyme binding capacity 45 mg/mk
pH stability 2-13
Filling quantity Delivered as a 50 % suspension
Matrix 7.5% cross-linked agarose
Short Term Storage In neutral buffer at 4°C
Long Term Storage 20 mM sodium acetate, 20% ethanol, pH 6.5 at 4 °C
Chemical Structure of Butyl ligand Butyl group structure

Video

Video Guide - Hydrophobic Interaction Chromatography (Jump to 8:04)
Video Guide - How to pack FPLC cartridges

FAQ

Can I get the datasheet for the PureCube Butyl Agarose?

What can I do with these beads?

The beads are meant for Hydrophobic Interaction Chromatography or HIC for short. This purification method separates proteins and other biomolecules based on their hydrophobicity. How the process works in detail can be found in THIS VIDEO GUIDE