PureCube Fe-NTA Magbeads / Magnetic beads were developed for the enrichment of phosphorylated proteins. They can also be used for the purification of his tagged proteins as a secondary function. The affinity matrix is based on 6% cross-linked agarose that was magnetized. The bead ligand is Fe-NTA and the particle diameter is 30 µm. They are very homogeneous in size, yielding in a high degree of reproducibility. The phosphopeptide enrichment can be automated as it has already been proven in this paper. As an alternative we also offer PureCube Fe-NTA Agarose resin and said resin pre-packed in cartridges/columns.
Features - Regarding Purification capabilities.
Feature
Usage
Enrichment of phosphopeptides
Specific binding and purification of 6x his-tagged proteins
Specifity
Affinity to His-tagged proteins
Affinity to phosphorylated biomolecules
Bead Ligand
Fe-NTA
Bead size
30 µm
Filling quantity
Delivered as a 25 % suspension
Chelator stability
Stable in buffer containing 10 mM DTT and 1 mM EDTA
pH stability
2-14
Other stabilities
100% methanol, 100% ethanol, 8 M urea, 6 M guanidinium hydrochloride, 30% (v/v) acetonitrile
