Rho Starter Set 1: PureCube Rho1D4 Agarose (1 ml) + Rho1D4 peptide (5 mg)

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Changing to a new affinity tag after working with another one for a certain amount of time... more
Product information "Rho Starter Set 1: PureCube Rho1D4 Agarose (1 ml) + Rho1D4 peptide (5 mg)"
Type: Agarose
Bead size: 40 µm
Changing to a new affinity tag after working with another one for a certain amount of time can be unappealing to some. However if the new affinity tag out matches the previously used one in both protein yield and protein purity, every responsible scientists knows how to act. See our compairison table between the FLAG-tag and Rho1D4-tag as a prime example for this.
To provide your lab with an affordable option to quickly switch to the Rho1D4-tag we offer our Rho1D4 purification protducts in practical starter sets together with the corresponding elution peptide for a reduced price.

Our Rho1D4 Starter Set 1 combines our high-quality PureCube Rho1D4 affinity agarose resin with the Rho1D4 elution peptide for your convenience.
rho1d4 tag membrane protein Schematic of a membrane protein tagged with Rho1D4

Features

 
Usage Highly specific affinity purification of Rho1D4 (TETSQVAPA) tagged proteins
Specifity Affinity to Rho1D4-tagged proteins
Special feature Highly recommended for the purification of membrane proteins.
Binding capacity 3-4 mg / ml Resin
Bead Ligand Rho1D4 Antibody
Bead size 40 μm
Chelator stability Stable in buffer containing 10 mM DTT and 1 mM EDTA
Filling quantity Delivered as a 50 % suspension
Required equipment
 
  • Lysis Buffer
  • Wash Buffer
  • Elution Buffer
  • Ice bath
  • Refrigerated centrifuge for 50 mL tube (min 10,000 x g)
  • 50 mL centrifuge tube
  • Micropipettor and Micropipetting tips
  • Disposable gravity flow columns with capped bottom outlet, 2 ml
  • pH meter
  • End-over-end shaker
  • SDS-PAGE buffers, reagents and equipment Optional: Western Blot reagents and equipment

Information about Rho1D4 elution peptide

Features

Usage Elution of Rho1D4 tagged proteins during the elution step of a protein purification procedure.
Specifity Affinity to Rho1D4 affinity beads
Sequence Ac_TETSQVAPA_ NH2
Amount 5 mg, lyophilized
Storage, short term Store lyophilized and reconstituted peptide at 4°C
Storage, long term Store lyophilized and reconstituted peptide at-20°C
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