Rho Starter Set 2: HighSpec Rho1D4 MagBeads (1 ml) + HighSpec Rho1D4 peptide (5 mg)

Order number: 33299
€432.00*

€242.00*

Ready to ship today
Quantity

Description

Our Rho1D4 Starter Set 2 is a combination of our HighSpec Rho1D4 MagBeads and the Rho1D4 elution peptide. It is meant for protein purification assays of the highest possible purity, exploiting the specificity of an antibody to its antigen. More on the Rho1D4-tag can be read here.

Do you prefer agarose resin over magentic beads or would like to have the Rho1D4 agarose pre-packed into an FPLC column? No problem, as we also offer other Rho1D4 Starter Sets. Furthermore, we recommend our Rho1D4 antibody for later western blot detection of your protein.
Feature - Rho1D4 MagBeads
Usage Specific binding and purification of Rho1D4-tagged proteins
Specificity Affinity to Rho1D4-tagged (TETSQVAPA) proteins
Binding capacity >3 mg/ml
Bead Ligand anti-Rho1D4 antibody
Bead size 30 μm
Matrix 6% cross-linked agarose
Filling quantity Delivered as a 25 % suspension
Short-term storage In neutral buffer at 4°C
Long-term storage In neutral buffer with 20% ethanol at 4°C


Feature - Rho1D4 Agarose
Usage Elution of Rho1D4-tagged protein from matching purification beads
Specifity Affinity to Rho1D4 affinity beads
Sequence Ac_TETSQVAPA_NH2
Amount 5 mg per tube
Storage, short term Store lyophilized and reconstituted peptide at 4°C
Storage, long term Store lyophilized and reconstituted peptide at-20°C

Lab Results

Rho1D4-tag purification products are especially handy when it comes to the purification of proteins with low abundance. And a huge group of these proteins is membrane proteins. Even overexpressed their concentration does not come close to their soluble counterparts.

Figure 1 shows the purification of four different membrane proteins. Each time the purification has resulted in great purity and lots of protein.
Examples for RHo1D4-tag protein purifications using Cube Biotech products
Figure 1: Example of Rho1D4-tag purifications using Rho1D4 MagBeads. The four depicted anti-Rho1D4 western blots show one different mutant of the SARS-CoV-2 surface protein SPIKE. A: Alpha mutant B.1.1.7 B: Beta mutant B.1.351 C: Epsilon mutant B.1.429 D: Eta mutant B.1.525

FAQ

Can I get the datasheet for the HighSpec Rho1D4 MagBeads or Rho1D4 peptide?

Yes, Just click to download both here.
Rho1D4 MagBeads Datasheet
Rho1D4 Peptide Datasheet

Can I use the Rho1D4-tag also for non-membrane proteins?

Yes, you can.

I never heard about the Rho1D4-tag before. What can you tell me about it?

Do not worry. All your questions should be answered on our Rho1D4-tag knowledge page.

I use the FLAG-tag (DYKDDDDK) for my protein purifications. Is it beneficial for me to switch to the Rho1D4-tag?

Can I regenerate & reuse your Glutathione beads?