Rho Starter Set 3: HighSpec Compact Cartridge Rho1D4 (1x1 ml) + HighSpec Rho1D4 peptide (5 mg)

Order number: 33398
€486.00*

€267.00*

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Description

Our Rho1D4 Starter Set 3 is a combination of our HighSpec Rho1D4 Compact Cartridge and the Rho1D4 elution peptide. The Compact Cartridge itself is an FPLC column pre-packed with our HighSpec Rho1D4 agarose resin. It is meant for protein purification assays of the highest possible purity, exploiting the specificity of an antibody to its antigen. More on the Rho1D4-tag can be read here.

Do you prefer your agarose resin unpacked for e.g. column purification or magnetic beads, as we also offer other Rho1D4 Starter Sets. Furthermore, we recommend our Rho1D4 antibody for later western blot detection of your protein.
Feature - Pre-packed Rho1D4 Agarose
Usage Specific binding and purification of Rho1D4-tagged proteins
Specificity Affinity to Rho1D4-tagged (TETSQVAPA) proteins
Binding capacity >3 mg/ml
Bead Ligand anti-Rho1D4 antibody
Bead size 40 μm
Matrix 7.5% cross-linked agarose
Recommended flow rates 0.25 - 1 ml/min
Max. flow rates 6 ml/min
Filling quantity Delivered as a 50 % suspension
Short-term storage In neutral buffer at 4°C
Long-term storage In neutral buffer with 20% ethanol at 4°C
Required equipment & materials
  • FPLC hardware like e.g. AEKTA
  • Binding Buffer
  • Elution Buffer


Feature - Column
PureCube Compact Cartridge 1 mL PureCube Compact Cartridge 5 mL
Bed Volume 1 mL 5 mL
Max. Flow Rate 6 mL/min 6 mL/min
Dimensions: diameter X length (mm) 5 X 35 17 X 35
Body material Polypropylene Polypropylene
Inlet 10-32 UNF female thread 10-32 UNF female thread
Outlet 10-32 UNF female thread 10-32 UNF female thread


Feature - Rho1D4 Elution Peptide
Usage Elution of Rho1D4-tagged protein from matching purification beads
Specifity Affinity to Rho1D4 affinity beads
Sequence Ac_TETSQVAPA_NH2
Amount 5 mg per tube
Storage, short term Store lyophilized and reconstituted peptide at 4°C
Storage, long term Store lyophilized and reconstituted peptide at-20°C

Lab Results

Rho1D4-tag purification products are especially handy when it comes to the purification of proteins with low abundance. And a huge group of these proteins is membrane proteins. Even overexpressed their concentration does not come close to their soluble counterparts.

Figure 1 shows the purification of four different membrane proteins. Each time the purification has resulted in great purity and lots of protein.
Examples for RHo1D4-tag protein purifications using Cube Biotech products
Figure 1: Example of Rho1D4-tag purifications using Rho1D4 MagBeads. The four depicted anti-Rho1D4 western blots show one different mutant of the SARS-CoV-2 surface protein SPIKE. A: Alpha mutant B.1.1.7 B: Beta mutant B.1.351 C: Epsilon mutant B.1.429 D: Eta mutant B.1.525

FAQ

Can I get the datasheet for the HighSpec Rho1D4 Compact Cartridge or Rho1D4 peptide?

Yes, Just click to download both here.
Rho1D4 Compact Cartridge Datasheet
Rho1D4 Peptide Datasheet

Can I use the Rho1D4-tag also for non-membrane proteins?

Yes, you can.

I never heard about the Rho1D4-tag before. What can you tell me about it?

Do not worry. All your questions should be answered on our Rho1D4-tag knowledge page.

I use the FLAG-tag (DYKDDDDK) for my protein purifications. Is it beneficial for me to switch to the Rho1D4-tag?

Can I regenerate & reuse your Glutathione beads?