PureCube Rho1D4 Agarose XL

PureCube Rho1D4 Agarose XL
€277.00 *

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ML:

  • 57601
Rho1D4 , or 1D4 for short, is the name of a nine amino acid long affinity tag (TETSQVAPA),... more
Product information "PureCube Rho1D4 Agarose XL"
Type: Agarose
Bead size: 90 µm
Rho1D4 , or 1D4 for short, is the name of a nine amino acid long affinity tag (TETSQVAPA), which can be used for highly specific affinity protein purification. Its specialty is the capabilities for membrane protein purification. It even outperformes the more better known Flag-Tag in both purity and protein yield. Our PureCube Rho1D4 XL agarose resin was made for FPLC protein purification assays if normal sized beads are resulting in to low flow rated in FPLC assays.
It is composed of ~400 µm diameter agarose beads that are functionalized with the Rho1D4 antibody. This product is also available as MagBeads or regular sized agarose resin. We further recommend to use the Rho1D4 protein elution peptide instead of pH changes for the elution step of the purification procedure.
rho1d4 tag membrane protein Schematic of a membrane protein tagged with Rho1D4

Features

 
Usage Highly specific affinity purification of Rho1D4 (TETSQVAPA) tagged proteins
Specifity Affinity to Rho1D4 tagged proteins
Special feature Highly recommended for the purification of membrane proteins.
Binding capacity 3 mg / ml Resin
Bead Ligand Rho1D4 Antibody
Bead size 400 μm
Chelator stability Stable in buffer containing 10 mM DTT and 1 mM EDTA
Filling quantity Delivered as a 50 % suspension
Required equipment
 
  • Lysis Buffer
  • Wash Buffer
  • Elution Buffer
  • Ice bath
  • Refrigerated centrifuge for 50 mL tube (min 10,000 x g)
  • 50 mL centrifuge tube
  • Micropipettor and Micropipetting tips
  • Disposable gravity flow columns with capped bottom outlet, 2 ml
  • pH meter
  • End-over-end shaker
  • SDS-PAGE buffers, reagents and equipment Optional: Western Blot reagents and equipment
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