Cu-NTA

Cu-NTA is a term that refers to a copper ion that has been coupled Nitrilotriacetic acid (NTA). Cu-NTA can then be coupled to agarose or magnetic beads for Immobilized Metal Chelate Affinity Chromatography, short IMAC. This is a purification method for two purposes.

The purification of his tagged proteins
The enrichment of copper binding proteins

Cube Biotech offers multiple different Cu-NTA based products for your specific assay.

Overview of Cube Biotech's Cu-NTA products for protein purification.


Cu-NTA agarose (40 µm)	Cu-NTA agarose (40 µm) Cartridge	Cu-NTA Magnetic Beads
	Cu-NTA agarose (40 µm) COMPACT Cartridge

Features

Usage	Specific binding and purification of 6x his-tagged proteins
Specifity	Affinity to His-tagged proteins and copper-binding proteins
Bead size	40 µm (agarose resin) 30 µm (magnetic beads)
Binding capacity	Depending on the application
Metal Ion capacity	>12 µeqv Zn²⁺/mL.
Chelator stability	Stable in buffer containing 10 mM DTT and 1 mM EDTA
Bead Ligand	Cu-NTA

Cu-NTA products by Cube Biotech were successfully used in the following publications:

Protein	Year	Author	Product
HrpII	2017	Bauer W.S., Richardson K.A., Adams N.M., Ricks K.M., Gasperino D.J., Ghionea S.J., Rosen M., Nichols K.P., Weigl B.H., Haselton F.R., Wright D.W., ¹	Cu-NTA MagBeads

Different metal ions confer different binding affinity and specificity

Depending on the chelating metal ion the results of a His tag protein purification may change. The coupled metal ion influences the balance between protein affinity to the His tagged protein and the specificity of the purification. Copper as the chelating ion is the choice with the highest affinity. Using Copper-NTA will lead to the highest protein yield.

metal_ions_protein purification affinity

Fig. 1: Affinity and specificity of metal ions commonly used for IMAC. Loading an IMAC resin with different metal ions can adjust the affinity and specificity to optimize the purity and yield of a purified protein.

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