Cu-NTA


Purification Resins
Cu-NTA is a term that refers to a copper ion that has been coupled Nitrilotriacetic acid (NTA). Cu-NTA can then be coupled to agarose or magnetic beads for Immobilized Metal Chelate Affinity Chromatography, short IMAC. This is a purification method for two purposes.
  1. The purification of his tagged proteins
  2. The enrichment of copper binding proteins
Cube Biotech offers multiple different Cu-NTA based products for your specific assay.

Overview of Cube Biotech's Cu-NTA products for protein purification.


Features

Usage Specific binding and purification of 6x his-tagged proteins
Specifity Affinity to His-tagged proteins and copper-binding proteins
Bead size
  • 40 µm (agarose resin)
  • 30 µm (magnetic beads)
Binding capacity Depending on the application
Metal Ion capacity >12 µeqv Zn2+/mL.
Chelator stability Stable in buffer containing 10 mM DTT and 1 mM EDTA
Bead Ligand Cu-NTA

Cu-NTA products by Cube Biotech were successfully used in the following publications:

 ProteinYearAuthorProduct
HrpII 2017  Bauer W.S., Richardson K.A., Adams N.M., Ricks K.M., Gasperino D.J., Ghionea S.J., Rosen M., Nichols K.P., Weigl B.H., Haselton F.R., Wright D.W., 1 Cu-NTA MagBeads

Different metal ions confer different binding affinity and specificity

Depending on the chelating metal ion the results of a His tag protein purification may change. The coupled metal ion influences the balance between protein affinity to the His tagged protein and the specificity of the purification. Copper as the chelating ion is the choice with the highest affinity. Using Copper-NTA will lead to the highest protein yield.
metal_ions_protein purification affinity
Fig. 1: Affinity and specificity of metal ions commonly used for IMAC. Loading an IMAC resin with different metal ions can adjust the affinity and specificity to optimize the purity and yield of a purified protein.
Top seller
PureCube Compact Cartridge Cu-NTA PureCube Compact Cartridge Cu-NTA
Article number: 31602-Cu
PureCube Cu-NTA Agarose PureCube Cu-NTA Agarose
Article number: 31403-Cu
PureCube Cu-NTA MagBeads PureCube Cu-NTA MagBeads
Article number: 31501-Cu
PureCube Cu-NTA Cartridge PureCube Cu-NTA Cartridge
Article number: 31601-Cu
Cu-NTA
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PureCube Compact Cartridge Cu-NTA PureCube Compact Cartridge Cu-NTA
Pre-Filled FPLC COMPACT column with Cu-NTA Agarose. For high-yield His Tag purification and copper binding enrichment.
Article number: 31602-Cu
Sales price: From €37.00 *
PureCube Cu-NTA Agarose PureCube Cu-NTA Agarose
For Copper-Binding and for His-tagged protein. | Cu-NTA agarose for high affinity and yield. | Suited of FPLC!
Article number: 31403-Cu
Sales price: From €107.00 *
PureCube Cu-NTA MagBeads PureCube Cu-NTA MagBeads
For Copper-Binding and for His-tagged protein. | Cu-NTA agarose for highest protein yield of his tagged proteins.
Article number: 31501-Cu
Sales price: From €68.00 *
PureCube Cu-NTA Cartridge PureCube Cu-NTA Cartridge
Pre-Filled FPLC column with Copper-NTA Agarose. Perfect for high-yield His Tag purification and copper binding enrichment.
Article number: 31601-Cu
Sales price: From €37.00 *
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