PureCube Copper-NTA (Cu-NTA) agarose resin were developped by Cube Biotech for two purposes: First protein purification of his tagged protein with the highest possible yield. The second purpose is the enrichment of copper-binding proteins. By using magnetic beads / magbeads, even small assays can be performed with minimal setup. As an alternative to MagBeads we also offer Cu-NTA agarose resin for batch spin columns and FPLC We also offer ou this Cu-NTA resin pre-filled into FPLC cartridges / columns
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Features
Usage
Specific binding and purification of 6x his-tagged proteins Enrichment of copper binding proteins
Specifity
Affinity to His-tagged proteins Affinity to copper-binding proteins
Bead Ligand
Cu-NTA
Bead size
30 μm
pH stability/span>
2-14
Chelator stability
Stable in buffer containing 10 mM DTT and 1 mM EDTA
Other stabilities
100% methanol, 100% ethanol, 8 M urea, 6 M guanidinium hydrochloride, 30% (v/v) acetonitrile
