PureCube Compact Cartridge Cu-NTA
Order number: 31602-Cu
Description
The PureCube Cu-NTA Compact cartridge is an IMAC chromatography column prepacked with PureCube Cu-NTA resin. You can choose between two sizes, 1 mL bed volume and 5 mL bed volume. Please have a look at the given features table to ensure that the Cartridge / Column is compatible with your FPLC hardware like ÄKTA TM..
If this is not the case we recommend buying the sole loose PureCube Cu-NTA resin and packing the columns yourself. A dedicated tutorial can be found on the matching site.
Alternatively, we also offer our PureCube Cu-NTA products as magnetic beads, if you prefer this IMAC method.
If this is not the case we recommend buying the sole loose PureCube Cu-NTA resin and packing the columns yourself. A dedicated tutorial can be found on the matching site.
Alternatively, we also offer our PureCube Cu-NTA products as magnetic beads, if you prefer this IMAC method.
Datasheets
Feature - Cu-NTA resin | |
---|---|
Usage | Specific binding and purification of 6x His-tagged proteins Binding of Copper-binding proteins |
Specificity | Affinity to His-tagged proteins Affinity to copper-binding |
Binding capacity | >80 mg/mL |
Bead Ligand | Cu-NTA |
Bead size | 40 μm |
Chelator stability | Stable in buffer containing 10 mM DTT and 1 mM EDTA |
Filling quantity | Delivered as a 50 % suspension |
Required equipment & materials |
|
Feature - Column | ||
---|---|---|
PureCube Compact Cartridge 1 mL | PureCube Compact Cartridge 5 mL | |
Bed Volume | 1 mL | 5 mL |
Max. Flow Rate | 6 mL/min | 6 mL/min |
Dimensions: diameter X length (mm) | 5 X 35 | 17 X 35 |
Body material | Polypropylene | Polypropylene |
Inlet | 10-32 UNF female thread | 10-32 UNF female thread |
Outlet | 10-32 UNF female thread | 10-32 UNF female thread |
Lab Results
Different metal ions confer different binding affinity and specificity
Loading different metal ions to a resin results in differing affinity and specificity for a His-tagged protein. Generally, copper exhibits the highest protein yield of commonly used IMAC metal ions, leading to relatively lower purity. In searching for the optimal resin to purify a protein, it is recommended to explore different chelating ligands (IDA or NTA) and different metal ions.
Loading different metal ions to a resin results in differing affinity and specificity for a His-tagged protein. Generally, copper exhibits the highest protein yield of commonly used IMAC metal ions, leading to relatively lower purity. In searching for the optimal resin to purify a protein, it is recommended to explore different chelating ligands (IDA or NTA) and different metal ions.
Video
Video Guide - FPLC
FAQ
Can I get the datasheet for the Cu-NTA Compact Cartridge?
What are the reasons for non-specific binding?
Some protein can bind to Cu-NTA even without having a His-tag. But to a lesser extent. Washing with NaOH after elution of your protein of interest removes unspecific bound proteins from your resin.
I want to use a high concentration of EDTA and DTT. Is it possible to use Cu-NTA from Cube Biotech?
No, it is not recommended because nickel-ions are reduced with DTT or dissolved with EDTA. If you want to use high concentrations of EDTA and DTT you should use our Indigo resin.
Can Cu-NTA beads be stripped?
Yes, they can. However, we recommend using our pre-stripped NTA or IDA beads, to begin with.
After using DTT my resin changed color. How to regenerate it?
The DTT has probably destroyed your beads. Cu-NTA beads only have a very limited DTT tolerance. However, you can regenerate them to regain their functionality. Please read our detailed protocol for more information regarding this. It is linked above.
However, we would recommend using Ni-INDIGO products instead. They work with the same buffers and protocols as the Cu-NTA products but have a DTT tolerance of 20 mM.
However, we would recommend using Ni-INDIGO products instead. They work with the same buffers and protocols as the Cu-NTA products but have a DTT tolerance of 20 mM.