Zn-NTA

Zn-NTA is a term that refers to a zinc ion that has been coupled with nitrilotriacetic acid (NTA). Zn-NTA can then be coupled to agarose or magnetic beads for Immobilized Metal Chelate Affinity Chromatography, short IMAC. This is a purification method for two purposes:

  1. The purification of his-tagged proteins
  2. The enrichment of zinc finger proteins