PureCube Zn-NTA MagBeads

Order number: 31590-Zn

€4,766.00*

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Description

PureCube Zn-NTA magnetic beads / MagBeads were developed by Cube Biotech for His-tag protein purification. Our year-long experience in manufacturing agarose resin lead to the high yield of 50 mg protein per ml resin, which is leading in the market compared to other Ni-IDA suppliers.

Ni-IDA products by Cube Biotech are also available as agarose resin beads for FPLC and batch spin. For your convenience Cube Biotech also offers its own MagBead separator for use with our magnetic beads.
Feature
Usage Specific binding and purification of 6x His-tagged proteins
Binding of Zinc-binding proteins
Specificity Affinity to His-tagged proteins
Affinity to Zinc-finger proteins
Binding capacity >50 mg/mL
Bead Ligand Zn-NTA
Bead size 30 μm
Filling quantity Delivered as a 25 % suspension
Metal ion capacity > 15 µeqv Zn2+/ml
pH stability 2-14
Other stabilities 100% methanol, 100% ethanol, 8 M urea, 6 M guanidinium hydrochloride, 30% (v/v) acetonitrile

Lab Results

Immobilized metal affinity chromatography (IMAC) is a popular method for protein purification, particularly for his-tagged recombinant proteins. But IMAC can be used in many other applications. Its high binding capacity towards the malaria biomarker protein HRP2 makes it an excellent enrichment method to increase sensitivity in malaria point-of-care diagnostic assays.

David W. Wright and his team at Vanderbilt University, Nashville, TN, USA have developed a novel device that efficiently combines proven point-of-care lateral flow assays with IMAC magnetic bead enrichment, which is deployed in collaboration with the Macha Research Trust in Zambia. In their work, Cube Biotech's Zn-NTA MagBeads showed highest biomarker protein recovery at lowest cost.

Cube Biotech Zn-NTA beads help to fullfill the ASSURED requirements of the WHO for malaria testing
  • A: Affordable
  • S: Sensetive
  • S: Specific
  • U: Rapid and Robust
  • R: Equipment-free
  • E: Deliverable
Malaria


Zn-NTA bead costs for Malaria diagnatic compared between competitors
Figure 1: Cube Biotech's Zn-NTA MagBeads provides most efficient HRP2 capture at lowest cost. Comparable volumes of IMAC MagBeads from four different manufacturers were evaluated as described in the application note.
Different metal ions confer different binding affinity and specificity

Loading different metal ions to a resin results in differing affinity and specificity for a His-tagged protein. Generally, zinc exhibits the second highest binding specificity of commonly used IMAC metal ions, leading to relatively lower yields but higher purity. In searching for the optimal resin to purify a protein, it is recommended to explore different chelating ligands (IDA or NTA) and different metal ions.
Metal ions compared in their performance for His-tag purifications
Figure 2: Affinity and specificity of metal ions commonly used for IMAC. Loading an IMAC resin with different metal ions can adjust the affinity and specificity to optimize the purity and yield of a purified protein.

Video

Video Guide - How to use MagBeads


FAQ

Can I get the datasheet for the Zn-NTA MagBeads?

What are the reasons for non-specific binding?

There are some protein that can bind to Zn-NTA even without having a His-tag. But to a lesser extent. Washing with NaOH after elution of your protein of interest removes unspecific bound proteins from your resin.

I want to use a high concentration of EDTA and DTT. Is it possible to use Zn-NTA from Cube Biotech?

No, it is not recommended because nickel-ions are reduced with DTT or dissolved with EDTA. If you want to use high concentrations of EDTA and DTT you should use our Indigo resin.

Can Zn-NTA beads be stripped?

Yes, they can. However, we recommend to use our pre-stripped NTA or IDA beads to begin with.

After using DTT my resin changed color. How to regenerate it?

The DTT has probably destroyed your beads. Zn-NTA beads only have a very limited DTT tolerance. However, you can regenerate them to regain their functionality. Please read our detailed protocol for more information regarding this. It is linked above.

However, we would recommend using Ni-INDIGO products instead. They work with the same buffers and protocols as the Ni-NTA products but have a DTT tolerance of 20 mM.