PureCube Compact Cartridge Zn-NTA

Order number: 31602-Zn

€52.00*

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Description

The PureCube Zn-NTA Compact cartridge is an IMAC chromatography column prepacked with PureCube Zn-NTA resin. You can choose between two sizes, 1 mL bed volume and 5 mL bed volume. Please have a look at the given features table to ensure that the Cartridge / Column is compatible with your FPLC hardware. Common systems like ÄKTA TM are competible without problem..
If this is not the case we recommend buying the sole loose PureCube Zn-NTA resin and packing the columns yourself. A dedicated tutorial can be found on the matching site.

Alternatively, we also offer our PureCube Zn-NTA products as magnetic beads, if you prefer this IMAC method.
Feature - Zn-NTA resin
Usage Specific binding and purification of 6x His-tagged proteins
Binding of Zinc-binding proteins
Specificity Affinity to His-tagged proteins
Affinity to Zinc-finger proteins
Binding capacity >50 mg/mL
Bead Ligand Zn-NTA
Bead size 40 μm
Chelator stability Stable in buffer containing 10 mM DTT and 1 mM EDTA
Filling quantity Delivered as a 50 % suspension
Required equipment & materials
  • FPLC hardware like e.g. AEKTA
  • Binding Buffer
  • Elution Buffer


Feature - Column
PureCube Compact Cartridge 1 mL PureCube Compact Cartridge 5 mL
Bed Volume 1 mL 5 mL
Max. Flow Rate 6 mL/min 6 mL/min
Dimensions: diameter X length (mm) 5 X 35 17 X 35
Body material Polypropylene Polypropylene
Inlet 10-32 UNF female thread 10-32 UNF female thread
Outlet 10-32 UNF female thread 10-32 UNF female thread

Lab Results

Immobilized metal affinity chromatography (IMAC) is a popular method for protein purification, particularly for his-tagged recombinant proteins. But IMAC can be used in many other applications. Its high binding capacity towards the malaria biomarker protein HRP2 makes it an excellent enrichment method to increase sensitivity in malaria point-of-care diagnostic assays.

David W. Wright and his team at Vanderbilt University, Nashville, TN, USA have developed a novel device that efficiently combines proven point-of-care lateral flow assays with IMAC magnetic bead enrichment, which is deployed in collaboration with the Macha Research Trust in Zambia. In their work, Cube Biotech's Zn-NTA MagBeads showed highest biomarker protein recovery at lowest cost.

Cube Biotech Zn-NTA beads help to fullfill the ASSURED requirements of the WHO for malaria testing
  • A: Affordable
  • S: Sensetive
  • S: Specific
  • U: Rapid and Robust
  • R: Equipment-free
  • E: Deliverable
Malaria


Zn-NTA bead costs for Malaria diagnatic compared between competitors
Figure 1: Cube Biotech's Zn-NTA MagBeads provides most efficient HRP2 capture at lowest cost. Comparable volumes of IMAC MagBeads from four different manufacturers were evaluated as described in the application note.
Different metal ions confer different binding affinity and specificity

Loading different metal ions to a resin results in differing affinity and specificity for a His-tagged protein. Generally, zinc exhibits the second highest binding specificity of commonly used IMAC metal ions, leading to relatively lower yields but higher purity. In searching for the optimal resin to purify a protein, it is recommended to explore different chelating ligands (IDA or NTA) and different metal ions.
Metal ions compared in their performance for His-tag purifications
Figure 2: Affinity and specificity of metal ions commonly used for IMAC. Loading an IMAC resin with different metal ions can adjust the affinity and specificity to optimize the purity and yield of a purified protein.

Video

Video Guide - FPLC

FAQ

Can I get the datasheet for the Zn-NTA compact cartridge?

What are the reasons for non-specific binding?

There are some protein that can bind to Zn-NTA even without having a His-tag. But to a lesser extent. Washing with NaOH after elution of your protein of interest removes unspecific bound proteins from your resin.

I want to use a high concentration of EDTA and DTT. Is it possible to use Zn-NTA from Cube Biotech?

No, it is not recommended because nickel-ions are reduced with DTT or dissolved with EDTA. If you want to use high concentrations of EDTA and DTT you should use our Indigo resin.

Can Zn-NTA beads be stripped?

Yes, they can. However, we recommend to use our pre-stripped NTA or IDA beads to begin with.

After using DTT my resin changed color. How to regenerate it?

The DTT has probably destroyed your beads. Zn-NTA beads only have a very limited DTT tolerance. However, you can regenerate them to regain their functionality. Please read our detailed protocol for more information regarding this. It is linked above.

However, we would recommend using Ni-INDIGO products instead. They work with the same buffers and protocols as the Zn-NTA products but have a DTT tolerance of 20 mM.