PureCube HiCap StrepTactin Cartridge / Column

Preview: PureCube HiCap StrepTactin Cartridge

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PureCube HiCap StrepTactin Cartridges / Columns were developed with the goal to provide a... more

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Product information "PureCube HiCap StrepTactin Cartridge"

Type:	Cartridge
Bead size:	100 µm

PureCube HiCap StrepTactin Cartridges / Columns were developed with the goal to provide a time-saving option for Strep®-tag based protein purifications for your lab. We achieved this by pre-filling our high quality PureCube HiCap StrepTactin Agarose resin into practical FPLC cartridges & columns. The cartridges were designed to be compatible for most FPLC hardware. However we recommend you to have a look at the given "features" table to confirm the compability to your hardware. For the rare cases that your FPLC hardware is imcompatible, please refer to our PureCube HiCap StrepTactin COMPACT Cartridges / Columns.

Features - Regarding Purification capabilities.

Usage	Highly specific affinity purification of Strep®-(WSHPQFEK)tagged proteins
Specifity	Affinity to Strep®-tagged proteins (WSHPQFEK)
Binding capacity	5 mg / ml Resin
Bead Ligand	StrepTactin ®
Bead size	100 μm
Stability	2% Tween 20, 2% Triton X-100, 2% IGEPAL® 630/Nonidet P40, n-Octyl-beta-D-glucopyranoside, 0,2% n-Nonyl-beta-D-gluyopyranoside, 0,35% N-Decyl-beta-D-maltoside, 2% Lauryl-sacrosine, 0,1% SDS, 0,3% CHAPS, 1M Guanidine HCl, 1mM PMSF, 10% Ethanol, 5M NaCl, 2M (NH₄)SO₄, 1M CaCl₂, 25% Glycerol
Filling quantity	Delivered as a 50 % suspension
Required equipment	Lysis Buffer Wash Buffer Elution Buffer Ice bath Refrigerated centrifuge for 50 mL tube (min 10,000 x g) 50 mL centrifuge tube Micropipettor and Micropipetting tips Disposable gravity flow columns with capped bottom outlet, 2 ml pH meter End-over-end shaker SDS-PAGE buffers, reagents and equipment Optional: Western Blot reagents and equipment

Features - Regarding Cartridge / Column itself.

Feature	PureCube Compact Cartridge 1 mL	PureCube Compact Cartridge 5 mL
Bed Volume	1 mL	5 mL
Dimensions: diameter X length (mm)	6.2 x 50	11 x 80
Body material	Polypropylene	Acrylate
End Plug Material	Polypropylene
Inlet	10-32 UNF female thread
Outlet	10-32 UNF female thread
Max. Flow Rate	6 mL/min

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