Strep antibody (0.1 mg)

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Order number: 40070

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Primary antibodies are often essential components of most experiments. And the Strep-Tag... more

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Product information "Strep antibody (0.1 mg)"

Type:

Antibody

Primary antibodies are often essential components of most experiments. And the Strep-Tag is one of the most commonly used affinity tags. Therefore we are providing a high quality primary antibody for this tag. Perfectly suited for chromogenic and chemiluminescence western blots.
We further recommend to use this antibody in combination with our high quality strep tag affiniy purification products.
As an alternative to this antibody we also offer the anti-strep HRP antibody.

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Features

Lot #	Varies
Antibody Name	Strep antibody
Cell Line	Mouse Hybridoma Produced using Strep-tag II peptide conjugated to keyhole limpet hemocyanin as immunogen
Subclass	IgG
Suitable for	Western blot, ELISA, Immune fluorescence
Form	100 μg lyophilized from NH₄CO₃, contains 10 mg sucrose per mg protein
Reconstitution of lyophilized antibody	Reconstitute antibody in pphosphate buffered saline (PBS) at cencentrations between 0.2 and 1.0 mg/ml (i.e. dissovle with 0.5 to 0.1 ml PBS)
Recommended dilution for Western Blot, chromogenic detection	0.2-0.5 μg/ml antibody diluted in PBS containing 2% BSA. Use rabbit anti-mouse conjugated to peroxidase as secondary antibody.
Recommended dilution for Western Blot, chemiluminescence detection	20 ng/ml antibody diluted in PBS
Reconstitution of lyophilized antibody	Add 500 µl ddH₂O to obtain a solution of 0.2 mg/ml concentration
Recommended dilution for FACS	10 μg/ml diluted in PBS, using e.g. goat anti mouse IgG AB conjugated to Cy2 (GE Healthcare, 1:400 dilution)
Recommended dilution for IF	10 μg/ml – 5 μg/ml antibody diluted in PBS (use secondary anti-mouse antibody)
Shipment Temperature	Shipped at room temperature (RT)
Storage	Store as lyophilized powder for up to 12 months at 2-8°C Alternatively, aliquot contents and freeze at -20°C for up to 6 months. Avaoid repeated freeze-thaw cycles.