
- Order number: 40070
Primary antibodies are often essential components of most experiments. And the Strep-Tag... more
Product information "Strep antibody (0.1 mg)"
Type: | Antibody |
Primary antibodies are often essential components of most experiments. And the Strep-Tag is one of the most commonly used affinity tags. Therefore we are providing a high quality primary antibody for this tag. Perfectly suited for chromogenic and chemiluminescence western blots.
We further recommend to use this antibody in combination with our high quality strep tag affiniy purification products.
As an alternative to this antibody we also offer the anti-strep HRP antibody.
We further recommend to use this antibody in combination with our high quality strep tag affiniy purification products.
As an alternative to this antibody we also offer the anti-strep HRP antibody.
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Features
Lot # | Varies |
Antibody Name | Strep antibody |
Cell Line | Mouse Hybridoma Produced using Strep-tag II peptide conjugated to keyhole limpet hemocyanin as immunogen |
Subclass | IgG |
Suitable for | Western blot, ELISA, Immune fluorescence |
Form | 100 μg lyophilized from NH4CO3, contains 10 mg sucrose per mg protein |
Reconstitution of lyophilized antibody | Reconstitute antibody in pphosphate buffered saline (PBS) at cencentrations between 0.2 and 1.0 mg/ml (i.e. dissovle with 0.5 to 0.1 ml PBS) |
Recommended dilution for Western Blot, chromogenic detection | 0.2-0.5 μg/ml antibody diluted in PBS containing 2% BSA. Use rabbit anti-mouse conjugated to peroxidase as secondary antibody. |
Recommended dilution for Western Blot, chemiluminescence detection | 20 ng/ml antibody diluted in PBS |
Reconstitution of lyophilized antibody | Add 500 µl ddH2O to obtain a solution of 0.2 mg/ml concentration |
Recommended dilution for FACS | 10 μg/ml diluted in PBS, using e.g. goat anti mouse IgG AB conjugated to Cy2 (GE Healthcare, 1:400 dilution) |
Recommended dilution for IF | 10 μg/ml – 5 μg/ml antibody diluted in PBS (use secondary anti-mouse antibody) |
Shipment Temperature | Shipped at room temperature (RT) |
Storage | Store as lyophilized powder for up to 12 months at 2-8°C Alternatively, aliquot contents and freeze at -20°C for up to 6 months. Avaoid repeated freeze-thaw cycles. |
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