A primary antibody is often an essential component of an experiments. And the Strep-Tag is one of the most popular affinity tags. Therefore Cube Biotech provides a high quality primary antibody for this tag. Suited for both chemiluminescence and chromogenic western blots. We especially recommend to use this antibody in tandem with our strep tag affiniy protein purification products. As an alternative to the HRP antibody we also offer the anti-strep antibody.
Suspension in PBS containing 5 mg/ml bovine serum albumin; approx. 0.05% ProClin 300 and stabilizing agents
Suitable for
Western blot, ELISA, Immune fluorescence
Form
100 μg lyophilized from NH4CO3, contains 10 mg sucrose per mg protein
Specificity
Strep-tag® II; the antibody was produced in mouse using Strep-tag® II peptide conjugated to keyhole limpet hemocyanin as immunogen
Detections
N- or C-terminal Strep-tag® II and Twin-Streptag ®
Dilutions
Dilute 1:10000 or higher for detection of Streptag ® II fusion proteins coated to microplates. Dilute 1:4000 for chromogenic and 1:32000 for chemiluminescence detection of Strep-tag® fusion proteins after Western blotting
