ACE2 Receptor (Ectodomain)

Order number: 28621

€306.00*

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Description

ACE2 (Angiotensin-converting enzyme 2) is the gateway of the coronavirus SARS-CoV-2 into our cells. Its actual cellular function is that of a cell surface-bound carboxypeptidase. We provide you with the Ectodomain of ACE2, ready for ligand binding studies, crystallization, and other biochemical/biophysical experiments.

If you are interested in the full-length ACE2 receptor contact our protein services. Or use this contact form to get in touch with us directly.
ACE2 in Coronavirus Infection scheme
Figure 1: ACE2 and its role in the SARC-CoV-2 infection.
Feature
Alternative names ACE2 (Angiotensin-converting enzyme 2)
UniProt number Q9BYF1
Protein class Single span transmembrane protein, Ectodomain
Organism Human (Homo sapiens)
Sequence, explained Ectodomain (aa 1 – 740), including signal peptide, 6 x His tag fused to C-terminus
Sequence, One-Letter Code MSSSSWLLLS LVAVTAAQST IEEQAKTFLD KFNHEAEDLF
YQSSLASWNY NTNITEENVQ NMNNAGDKWS AFLKEQSTLA
QMYPLQEIQN LTVKLQLQAL QQNGSSVLSE DKSKRLNTIL
NTMSTIYST GKVCNPDNPQ ECLLLEPGLN EIMANSLDYN
ERLWAWESWR SEVGKQLRPL YEEYVVLKN EMARANHYED
YGDYWRGDYE VNGVDGYDYS RGQLIEDVEH TFEEIKPLYE
HLHAYVRAK LMNAYPSYIS PIGCLPAHLL GDMWGRFWTNL
YSLTVPFGQKP NIDVTDAMVD QAWDAQRIF KEAEKFFVSV
GLPNMTQGFWE NSMLTDPGNV QKAVCHPTAW DLGKGDFRIL
MCTKVTMDD FLTAHHEMGH IQYDMAYAAQP FLLRNGANEG
FHEAVGEIMS LSAATPKHLK SIGLLSPDF QEDNETEINF
LLKQALTIVG TLPFTYMLE KWRWMVFKG EIPKDQWMKK
WWEMKREIV GVVEPVPHD ETYCDPASLF HVSNDYSFIR
YYTRTLYQFQ FQEALCQAA KHEGPLHKC DISNSTEAGQ
KLFNMLRLGK SEPWTLALE NVVGAKNMNV RPLLNYFEPL
FTWLKDQNK NSFVGWSTDW SPYADQSIKV RISLKSALGD
KAYEWNDNEM YLFRSSVAYA MRQYFLKVKN QMILFGEED
VRVANLKPRI SFNFFVTAPK NVSDIIPRTE VEKAIRMSRS
RINDAFRLND NSLEFLGIQ PTLGPPNQPP VSHHHHHH
Affinity tag C-terminal 6x His tag without spacer
Expression Host Hek293 Expi cells
Size 746 amino acids (including 6 x His tag)
86,1 kDa
Buffer composition 20 mM Hepes pH 7.4; 100 mM NaCl
Function Host cell receptor for virus entry, Protease
Preparation
  • Expression system: Hek293 Expi cells
  • Purification: Affinity purification (INDIGO Agarose) and size exclusion chromatography
  • Form: Liquid
  • Buffer composition: 20 mM Hepes pH 7.4; 100 mM NaCl
Quality Control
  • Purity: >95% as determined by SDS-PAGE
  • Homogeneity: Size Exclusion Chromatography
  • Activity: not determined

Lab Results

ACE2 westernblots
Figure 2: SDS-PAGE and subsequent western blot showing the Ectodomain of ACE2. The His-tagged protein was purified using INDIGO-Ni agarose.
ACE2 ELISA
Figure 3: The ACE2 ectodomain was immobilized at a concentration of 500 ng per well, the trimeric CoV-2 spike protein was used in triplicates at concentrations between 1 x 10-9M and 2 x 10-5M. The apparent KD value is approx. 2x 10-8M.

FAQ

Can I get the datasheet for the ACE2 receptor (Ectodomain)?

Do you also offer the full-length version of ACE2?

No as part of our standard product portfolio. But you can request it through our protein services.