Bacteriorhodopsin (Hs)


Proteins
Bacteriorhodopsin (BR) is a 7-transmembrane protein that acts as a light-driven proton pump in archaebacteria. Due to its covalently bound colored ligand, BR is widely used as a control membrane protein, for example for crystallization assays and biochemical/biophysical experiments. We provide BR of high purity, prepared as purple membrane patches from Halobacterium salinarum.

Bacteriorhodopsin_SDS-Page; BR_SDS-Page
Figure 1: SDS-PAGE of bacterio-rhodopsin in purple membrane patches from H. salinarum.
Bacteriorhodopsin_spectrum;absorbance
Figure 2: Absorbance spectrum of bacteriorhodopsin in purple membrane patches from H. salinarum.

 

Features

Protein Bacteriorhodopsin (BR, HsBR, bacteriopsin)
UniProt number P02945
Protein class 7-transmembrane protein
Organism Halobacterium salinarum S9
Sequence

Full-length, wildtype sequence N-terminal signal sequence (underlined) which is mostly absent in the preparation


MLELLPTAVE GVSQAQITGR PEWIWLALGT ALMGLGTLYF LVKGMGVSDP DAKKFYAITT LVPAIAFTMY LSMLLGYGLT MVPFGGEQNP IYWARYADWL FTTPLLLLDL ALLVDADQGT ILALVGADGI MIGTGLVGAL TKVYSYRFVW WAISTAAMLY ILYVLFFGFT SKAESMRPEV ASTFKVLRNV TVVLWSAYPV VWLIGSEGAG IVPLNIETLL FMVLDVSAKV GFGLILLRSR AIFGEAEAPE PSAGDGAAAT SD

Affinity tags None
Size (excluding additional elements) 262 amino acids; 28,256 Da
Expression system Halobacterium salinarum S9
Purified via Sequential centrifugation
Buffer Purple membrane (PM) patches in H2O, 0.02% NaN3
Purity (SDS-PAGE) >98%, see Fig. 1
Absorbance Extinction coefficient at 560 nm: 63,000 M-1 cm-1.
Activity Binding of ligand all-trans retinal, covalently bound to a lysine residue. Evaluation of UV-VIS spectrum: absorbance ratio 280 nm / 560 nm = 2.3
Function Photoreceptor protein undergoing a photocycle, Retinal protein, light-driven proton pump
Literature references
  1. Kubicek, Jan, et al. "Controlled in meso phase crystallization–a method for the structural investigation of membrane proteins." PloS one 7.4 (2012): e35458.
  2. Zscherp, Christian, Ramona Schlesinger, and Joachim Heberle. "Time-resolved FT-IR spectroscopic investigation of the pH-dependent proton transfer reactions in the E194Q mutant of bacteriorhodopsin." Biochemical and biophysical research communications 283.1 (2001): 57-63.
Proteins
Bacteriorhodopsin (BR) is a 7-transmembrane protein that acts as a light-driven proton pump in archaebacteria. Due to its covalently bound colored ligand, BR is widely used as a control membrane protein, for example for crystallization assays and biochemical/biophysical experiments. We provide BR of high purity, prepared as purple membrane patches from Halobacterium salinarum.

Bacteriorhodopsin_SDS-Page; BR_SDS-Page
Figure 1: SDS-PAGE of bacterio-rhodopsin in purple membrane patches from H. salinarum.
Bacteriorhodopsin_spectrum;absorbance
Figure 2: Absorbance spectrum of bacteriorhodopsin in purple membrane patches from H. salinarum.

 

Features

Protein Bacteriorhodopsin (BR, HsBR, bacteriopsin)
UniProt number P02945
Protein class 7-transmembrane protein
Organism Halobacterium salinarum S9
Sequence

Full-length, wildtype sequence N-terminal signal sequence (underlined) which is mostly absent in the preparation


MLELLPTAVE GVSQAQITGR PEWIWLALGT ALMGLGTLYF LVKGMGVSDP DAKKFYAITT LVPAIAFTMY LSMLLGYGLT MVPFGGEQNP IYWARYADWL FTTPLLLLDL ALLVDADQGT ILALVGADGI MIGTGLVGAL TKVYSYRFVW WAISTAAMLY ILYVLFFGFT SKAESMRPEV ASTFKVLRNV TVVLWSAYPV VWLIGSEGAG IVPLNIETLL FMVLDVSAKV GFGLILLRSR AIFGEAEAPE PSAGDGAAAT SD

Affinity tags None
Size (excluding additional elements) 262 amino acids; 28,256 Da
Expression system Halobacterium salinarum S9
Purified via Sequential centrifugation
Buffer Purple membrane (PM) patches in H2O, 0.02% NaN3
Purity (SDS-PAGE) >98%, see Fig. 1
Absorbance Extinction coefficient at 560 nm: 63,000 M-1 cm-1.
Activity Binding of ligand all-trans retinal, covalently bound to a lysine residue. Evaluation of UV-VIS spectrum: absorbance ratio 280 nm / 560 nm = 2.3
Function Photoreceptor protein undergoing a photocycle, Retinal protein, light-driven proton pump
Literature references
  1. Kubicek, Jan, et al. "Controlled in meso phase crystallization–a method for the structural investigation of membrane proteins." PloS one 7.4 (2012): e35458.
  2. Zscherp, Christian, Ramona Schlesinger, and Joachim Heberle. "Time-resolved FT-IR spectroscopic investigation of the pH-dependent proton transfer reactions in the E194Q mutant of bacteriorhodopsin." Biochemical and biophysical research communications 283.1 (2001): 57-63.
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Bacteriorhodopsin (Hs)
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Hs_Bacteriorhodopsin (500 µg) Hs_Bacteriorhodopsin (500 µg)
Active, purified purple membrane patches from Halobacterium salinarium. 5x 100 microgram in solution. Shipped on dry ice - additional freight cost may apply.
Article number: 28903
Sales price: €375.00 *
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