SARS CoV-2 Spike protein nanodisc complex

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The SPIKE protein is the surface protien of SARS-CoV-2, more commonly known as the... more
Product information "SARS CoV-2 Spike protein nanodisc complex"
The SPIKE protein is the surface protien of SARS-CoV-2, more commonly known as the coronavirus. It interacts on the cell's surface with the serine protease TMPRSS2 and the surface receptor ACE2.
Native spike protein conists of a trimer that is based in the virus membrane. Therefore purifiy native & active SPIKE the trimer needs to be kept intact. Cube Biotech achieved this in three ways:
  1. MSP nanodiscs, as you can see here
  2. Detergent Mycelles
  3. Synthetic nanodiscs
You can also purchase all three options together as a part of our practical 3 in 1 SPIKE protein set.

PDF Downloads

 
SPIKE in MSP nanodisc, SDS Page Figure 1: SDS Page of purified SPIKE protein in MSP1D1 nanodiscs. The band that represents the MSP protein can also be seen.
 
SPIKE in MSP nanodisc, Gel filtration Figure 2: Gel filtration result of SPIKE in MSP nanodisc. The peak that represents spike is marked with a red arrow.

Features

 
Alternative names SPIKE_SARS2 Spike glycoprotein
UniProt number P0DTC2
Protein class Single span transmembrane protein
Organism Severe acute respiratory syndrome coronavirus 2 (2019-nCoV) (SARS-CoV-2)
Sequence
  • Full-length sequence (aa 1 – 1273),
  • furin cleavage site “RRAR” mutated to “GSAG”;
  • C-terminal Rho1D4 tag fused with spacer “GSSG” to protein sequence
Affinity tag C-terminal Rho1D4
Expression Host Hek293 Expi cells
Size 1286 amino acids (including Rho1D4 tag and linker)
142114 Da
Buffer composition 20 mM Hepes pH 7.5; 150 mM NaCl,
Nanodisc MSP1D1-His, assembly with POPC lipids
(Membrane scaffold protein based nanodisc; MSP1D1 = 25,3 kDa)
Function host cell surface receptor binding; fusion of virus membrane with host endosome membrane
Purity (SDS-PAGE) >98% as determined by SDS-PAGE, see Fig. 1 A and B
Homogeneity >Size exclusion chromatography, Superose 6 Increase, see Fig. 2
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