SARS CoV-2 spike protein in DIBMA Glycerol

€850.00 *

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µG:

  • 28706
SARS-CoV-2, more commonly known as the coronavirus, enters the cell by using its surface... more
Product information "SARS CoV-2 spike protein in DIBMA Glycerol"
SARS-CoV-2, more commonly known as the coronavirus, enters the cell by using its surface protein called SPIKE. SPIKE is processed on the cell's surface through the serine protease TMPRSS2 and subsequently binds to the surface receptor ACE2.
The native SPIKE protein is a trimer and is located in the coronavirus membrane. Therefore to obtain pure & native SPIKE the trimer needs to be kept intact. We achieved this in three different ways:
  1. MSP nanodiscs, based on MSP proteins
  2. Detergent Mycelles
  3. Synthetic nanodiscs, as you can see here
You can also purchase all three options together as a part of our practical 3 in 1 SPIKE protein set.

PDF Downloads

 
SPIKE in Synthetic Nanodisc, SDS page and Native Gel Figure 1: Data from our lab regarding SPIKE stabilized and purified in synthetic nanodiscs (Dibma Glycerol).
A: SDS of SPIKE in synthetic nanodisc.
B: Western blot of Rhot1d4 tagged SPIKE, originated from the SDS Page seen in "A"
C: Native protein Gel of purified and stabilized SPIKE in synthetic nanodisc.

Features

 
Alternative names SPIKE_SARS2 Spike glycoprotein
UniProt number P0DTC2
Protein class Single span transmembrane protein
Organism Severe acute respiratory syndrome coronavirus 2 (2019-nCoV) (SARS-CoV-2)
Sequence
  • Full-length sequence (aa 1 – 1273),
  • furin cleavage site “RRAR” mutated to “GSAG”;
  • C-terminal Rho1D4 tag fused with spacer “GSSG” to protein sequence
Affinity tag C-terminal Rho1D4
Expression Host Hek293 Expi cells
Size 1286 amino acids (including Rho1D4 tag and linker)
142114 Da
Buffer composition 20 mM Hepes pH 7.5; 150 mM NaCl,
buffer does not contain free DIBMA Glycerol (DIBMA: Diisobutylene-maleic acid)
Function host cell surface receptor binding; fusion of virus membrane with host endosome membrane
Purity (SDS-PAGE) >98% as determined by SDS-PAGE, see Fig. 1 A and B
Homogeneity Native Gel, see Fig. 1 C
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