SARS CoV-2 spike protein in LMNG detergent

€650.00 *

Prices plus VAT plus shipping costs

Ready to ship today,
Delivery time appr. 1-3 workdays

µG:

  • 28702
The coronavirus, also known as SARS-CoV-2, enters the cell by using its surface SPIKE. SPIKE... more
Product information "SARS CoV-2 spike protein in LMNG detergent"
The coronavirus, also known as SARS-CoV-2, enters the cell by using its surface SPIKE. SPIKE is processed on the cell's surface by TMPRSS2, a serine protease. It then subsequently binds to ACE2 a cell surface receptor.
The Native SPIKE protein is a trimer that is located in the coronavirus membrane. Therefore to get pure & native SPIKE the trimer needs to be kept intact. Our lab staff achieved this in three different ways:
  1. MSP nanodiscs, based on MSP proteins
  2. Detergent Mycelles, as you can see here
  3. Synthetic nanodiscs
You can also purchase all three options together as a part of our practical 3 in 1 SPIKE protein set.

PDF Downloads

 
SPIKE in Degergent, SDS page, westernblot and Native GelFigure 1: Data from our lab regarding SPIKE stabilized in a detergent mycelle.
A: SDS-Page of purified SPIKE in detergent mycelle.
B: Western blot of Rho1d4 tagged SPIKE, originated from the SDS Page seen in "A"
C: Native protein Gel of purified and stabilized SPIKE in detergent mycelles..

Features

 
Alternative names SPIKE_SARS2 Spike glycoprotein
UniProt number P0DTC2
Protein class Single span transmembrane protein
Organism Severe acute respiratory syndrome coronavirus 2 (2019-nCoV) (SARS-CoV-2)
Sequence
  • Full-length sequence (aa 1 – 1273),
  • furin cleavage site “RRAR” mutated to “GSAG”;
  • C-terminal Rho1D4 tag fused with spacer “GSSG” to protein sequence
Affinity tag C-terminal Rho1D4
Expression Host Hek293 Expi cells
Size 1286 amino acids (including Rho1D4 tag and linker)
142114 Da
Buffer composition 20 mM Hepes pH 7.5; 150 mM NaCl, 0.01 % LMNG
Function host cell surface receptor binding; fusion of virus membrane with host endosome membrane
Purity (SDS-PAGE) >98% as determined by SDS-PAGE, see Fig. 1 A and B
Homogeneity Native Gel, see Fig. 1 C
Related links to "SARS CoV-2 spike protein in LMNG detergent"
Rho1D4 antibody (0.2 mg) Rho1D4 antibody (0.2 mg)
Article number: 40020
Sales price: €279.00 *
Product Image SPIKE protein in DIBMA SARS CoV-2 spike protein in DIBMA Glycerol
Article number: 28706
Sales price: From €850.00 *
MSP1D1, lyophilized protein MSP1D1, lyophilized protein
Article number: 26132
Sales price: From €243.00 *
SARS CoV-2 spike protein set in LMNG detergent (100 µg), DIBMA Glycerol (25 µg) and nanodisc complex SARS CoV-2 spike protein set in LMNG detergent...
Article number: 28704
Sales price: €2,290.00 *
PureCube 100 INDIGO Ni-Cartridge PureCube 100 INDIGO Ni-Cartridge
Article number: 75301
Sales price: From €48.00 *
PureCube 100 Ni-NTA Agarose PureCube 100 Ni-NTA Agarose
Article number: 74103
Sales price: From €86.40 * €108.00 *
PureCube DIBMA 12, Screening Kit, 10x50 mg, HEPES PureCube DIBMA 12, Screening Kit, 10x50 mg, HEPES
Article number: 18011
Sales price: €152.15 * €179.00 *
PureCube 1-step batch Midi Plus PureCube 1-step batch Midi Plus
Article number: 63203
Sales price: From €47.00 *
PureCube 1-step batch Mini PureCube 1-step batch Mini
Article number: 63104
Sales price: From €104.00 *
PureCube NHS Activated MagBeads PureCube NHS Activated MagBeads
Article number: 50401
Sales price: From €110.00 *
PureCube Carboxy MagBeads PureCube Carboxy MagBeads
Article number: 50201
Sales price: From €85.00 *
PureCube Carboxy Agarose PureCube Carboxy Agarose
Article number: 50103
Sales price: From €87.00 *
PentaHis antibody, BSA-free (0.1 mg) PentaHis antibody, BSA-free (0.1 mg)
Article number: 40040
Sales price: €448.00 *
PureCube Butyl Agarose PureCube Butyl Agarose
Article number: 39003
Sales price: From €55.00 *
Hs Bacteriorhodopsin Hs Bacteriorhodopsin
Article number: 28903
Sales price: €383.00 *
Nanodisc Assembly Kit MSP1D1 dH5-His_POPC Nanodisc Assembly Kit MSP1D1 dH5-His_POPC
Article number: 26223
Sales price: €245.00 *
Nanodisc Assembly Kit MSP1D1-His_POPC Nanodisc Assembly Kit MSP1D1-His_POPC
Article number: 26213
Sales price: €245.00 *
MSP1D1-His, lyophilized protein MSP1D1-His, lyophilized protein
Article number: 26112
Sales price: From €114.30 * €127.00 *
Isopropyl beta-D-1-thiogalactopyranoside (IPTG) Isopropyl beta-D-1-thiogalactopyranoside (IPTG)
Article number: 16602
Sales price: From €72.00 *
Detergent Screening Set Phosphocholines 1 (1 g each of Fos-12, Fos-14, and Fos-16) Detergent Screening Set Phosphocholines 1 (1 g...
Article number: 16093
Sales price: €287.00 *
n-Nonyl-phosphocholine (Fos-choline-9) n-Nonyl-phosphocholine (Fos-choline-9)
Article number: 16049
Sales price: From €136.00 *
n-Hexadecyl-phosphocholine (Fos-choline-16) n-Hexadecyl-phosphocholine (Fos-choline-16)
Article number: 16037
Sales price: From €130.00 *
n-Tetradecyl-phosphocholine (Fos-choline-14) n-Tetradecyl-phosphocholine (Fos-choline-14)
Article number: 16029
Sales price: From €130.00 *
n-Tridecyl-phosphocholine (Fos-choline-13) n-Tridecyl-phosphocholine (Fos-choline-13)
Article number: 16025
Sales price: From €136.00 *
n-Dodecyl-phosphocholine (Fos-choline-12) n-Dodecyl-phosphocholine (Fos-choline-12)
Article number: 16021
Sales price: From €118.00 *
Octylglucopyranoside (OG) Octylglucopyranoside (OG)
Article number: 16002
Sales price: From €140.00 *
CubeCrystal 2-well MO Plates CubeCrystal 2-well MO Plates
Article number: 10101
Sales price: From €88.00 *
Viewed