SARS CoV-2 spike protein in LMNG detergent

€650.00 *

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µG:

  • 28702
The coronavirus, also known as SARS-CoV-2, enters the cell by using its surface SPIKE. SPIKE... more
Product information "SARS CoV-2 spike protein in LMNG detergent"
The coronavirus, also known as SARS-CoV-2, enters the cell by using its surface SPIKE. SPIKE is processed on the cell's surface by TMPRSS2, a serine protease. It then subsequently binds to ACE2 a cell surface receptor.
The Native SPIKE protein is a trimer that is located in the coronavirus membrane. Therefore to get pure & native SPIKE the trimer needs to be kept intact. Our lab staff achieved this in three different ways:
  1. MSP nanodiscs, based on MSP proteins
  2. Detergent Mycelles, as you can see here
  3. Synthetic nanodiscs
You can also purchase all three options together as a part of our practical 3 in 1 SPIKE protein set.

PDF Downloads

 
SPIKE in Degergent, SDS page, westernblot and Native GelFigure 1: Data from our lab regarding SPIKE stabilized in a detergent mycelle.
A: SDS-Page of purified SPIKE in detergent mycelle.
B: Western blot of Rho1d4 tagged SPIKE, originated from the SDS Page seen in "A"
C: Native protein Gel of purified and stabilized SPIKE in detergent mycelles..

Features

 
Alternative names SPIKE_SARS2 Spike glycoprotein
UniProt number P0DTC2
Protein class Single span transmembrane protein
Organism Severe acute respiratory syndrome coronavirus 2 (2019-nCoV) (SARS-CoV-2)
Sequence
  • Full-length sequence (aa 1 – 1273),
  • furin cleavage site “RRAR” mutated to “GSAG”;
  • C-terminal Rho1D4 tag fused with spacer “GSSG” to protein sequence
Affinity tag C-terminal Rho1D4
Expression Host Hek293 Expi cells
Size 1286 amino acids (including Rho1D4 tag and linker)
142114 Da
Buffer composition 20 mM Hepes pH 7.5; 150 mM NaCl, 0.001 % LMNG
Function host cell surface receptor binding; fusion of virus membrane with host endosome membrane
Purity (SDS-PAGE) >98% as determined by SDS-PAGE, see Fig. 1 A and B
Homogeneity Native Gel, see Fig. 1 C
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