SARS CoV-2 full-length Spike B.1.1.7 Mutation

€258.00 *

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All viruses undergo fast mutations and adapt quickly to the countermeasures that the immune... more
Product information "SARS CoV-2 full-length Spike B.1.1.7 Mutation"
Product Type: Mutant Spike Protein
Protein Sequence: Full-length
All viruses undergo fast mutations and adapt quickly to the countermeasures that the immune systems creates. SARS-CoV-2 of the COVID-19 pandemic is no exception to this. During the pandemic multiple mutant strains arose. To help the sciencific community to combat these mutants Cube Biotech offers the SPIKE protein of the mutants in full-length and active in their native trimeric form, stabilized with the LMNG detergent.

This is the spike protein of the mutant strain B.1.1.7, also commonly known as the "UK / Great Britain mutant". Its individual mutations are listed in the "features" table below.

Features

Alternative names SPIKE_SARS2 Spike glycoprotein
UniProt number P0DTC2
Protein class Single span transmembrane protein
Organism Severe acute respiratory syndrome coronavirus 2 (2019-nCoV) (SARS-CoV-2)
Mutation that differ from "original" SPIKE protein del 69-70
del 144
N501Y
A570D
D614G
P681H
T716I
Further modifications
  • furin cleavage site “RRAR” mutated to “GSAG”; KV986PP
  • C-terminal Rho1D4 tag fused with spacer “GSSG” to protein sequence
Affinity tag C-terminal Rho1D4
Expression Host Hek293 Expi cells
Size 1286 amino acids (including Rho1D4 tag and linker)
142114 Da
Buffer composition 20 mM Hepes pH 7.5; 150 mM NaCl, 0.001 % LMNG
Function host cell surface receptor binding; fusion of virus membrane with host endosome membrane
Purity (SDS-PAGE) >98% as determined by SDS-PAGE, see Fig. 1 A and B
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