SARS CoV-2 full-length Spike B.1.1.7 Mutation

All viruses undergo fast mutations and adept quickly to the countermeasures that the immune systems creates against them. SARS-CoV-2 of the COVID-19 pandemic is no exception here. During the pandemic multiple mutant strains arose. To help the science combat these mutants Cube Biotech offers the SPIKE protein of these mutants in full-length and active in its native trimeric form, stabilized with the LMNG detergent.

This is the spike protein of the mutant strain B.1.1.7, also commonly known as the "UK / Great Britain mutant". Its individual mutations are listed in the "features" table below.

Further SPIKE related products by Cube Biotech include:

1. SPIKE of mutant strain B.1.351, also known as the "South Africa mutant" in LMNG.
2. SPIKE of mutant strain P.1, also known as the "Brazil Mutant" in LMNG.
3. The "original" SPIKE protein in LMNG.
4. The ectodomain of ACE2 - SPIKE's contact on the cell surface
5. Anti-SPIKE antibodies

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Figure 1: Data from our lab regarding SPIKE stabilized in a detergent mycelle.
A: SDS-Page of purified SPIKE in detergent mycelle.
B: Western blot of Rho1d4 tagged SPIKE, originated from the SDS Page seen in "A".

Features

Alternative names	SPIKE_SARS2 Spike glycoprotein
UniProt number	P0DTC2
Protein class	Single span transmembrane protein
Organism	Severe acute respiratory syndrome coronavirus 2 (2019-nCoV) (SARS-CoV-2)
Mutation that differ from "original" SPIKE protein	del 69-70 del 144 N501Y A570D D614G P681H T716I S982A D1118H
Further modifications	furin cleavage site “RRAR” mutated to “GSAG”; KV986PP C-terminal Rho1D4 tag fused with spacer “GSSG” to protein sequence
Affinity tag	C-terminal Rho1D4
Expression Host	Hek293 Expi cells
Size	1286 amino acids (including Rho1D4 tag and linker) 142114 Da
Buffer composition	20 mM Hepes pH 7.5; 150 mM NaCl, 0.001 % LMNG
Function	host cell surface receptor binding; fusion of virus membrane with host endosome membrane
Purity (SDS-PAGE)	>98% as determined by SDS-PAGE, see Fig. 1 A and B