All viruses undergo fast mutations and adept quickly to the countermeasures that the immune systems creates against them. SARS-CoV-2 of the COVID-19 pandemic is no exception here. During the pandemic multiple mutant strains arose. To help the science combat these mutants Cube Biotech offers the SPIKE protein of these mutants in full-length and active in its native trimeric form, stabilized with the LMNG detergent.
This is the spike protein of the mutant strain B.1.1.7, also commonly known as the "UK / Great Britain mutant". Its individual mutations are listed in the "features" table below.
A: SDS-Page of purified SPIKE in detergent mycelle.
B: Western blot of Rho1d4 tagged SPIKE, originated from the SDS Page seen in "A".
|Alternative names||SPIKE_SARS2 Spike glycoprotein|
|Protein class||Single span transmembrane protein|
|Organism||Severe acute respiratory syndrome coronavirus 2 (2019-nCoV) (SARS-CoV-2)|
|Mutation that differ from "original" SPIKE protein||del 69-70 |
|Further modifications|| |
|Affinity tag||C-terminal Rho1D4|
|Expression Host||Hek293 Expi cells|
|Size||1286 amino acids (including Rho1D4 tag and linker) |
|Buffer composition||20 mM Hepes pH 7.5; 150 mM NaCl, 0.001 % LMNG|
|Function||host cell surface receptor binding; fusion of virus membrane with host endosome membrane|
|Purity (SDS-PAGE)||>98% as determined by SDS-PAGE, see Fig. 1 A and B|