SARS CoV-2 full-length Spike B.1.429 Mutation

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All viruses undergo fast mutations and adapt quickly to the countermeasures that the immune... more
Product information "SARS CoV-2 full-length Spike B.1.429 Mutation"
Product Type: Mutant Spike Protein
Protein Sequence: Full-length
All viruses undergo fast mutations and adapt quickly to the countermeasures that the immune systems creates. SARS-CoV-2 of the COVID-19 pandemic is no exception to this. During the pandemic multiple mutant strains arose. To help the sciencific community to combat these mutants Cube Biotech offers the SPIKE protein of the mutants in full-length and active in their native trimeric form, stabilized with the LMNG detergent.

This is the spike protein of the mutant strain B.1.429, also known as mutant strain CAL.20C or "California mutant". Its individual mutations are listed in the "features" table below.
Further SPIKE related products by Cube Biotech include:
  1. SPIKE of mutant strain B.1.1.7, also known as the "UK mutant" in LMNG.
  2. SPIKE of mutant strain B.1.351, also known as the "South Africa mutant" in LMNG.
  3. SPIKE of mutant strain B.1.525, in LMNG.
  4. SPIKE of mutant strain P.1, also known as the "Brazil Mutant" in LMNG.
  5. The "original" SPIKE protein in LMNG.
  6. The ectodomain of ACE2 - SPIKE's contact on the cell surface
  7. Anti-SPIKE antibodies

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Uk mutant SPIKE in SDS page, westernblotFigure 1: Data from our lab regarding SPIKE stabilized in a detergent mycelle.
A: SDS-Page of purified SPIKE in detergent mycelle.
B: Western blot of Rho1d4 tagged SPIKE, originated from the SDS Page seen in "A".

Features

Alternative names SPIKE_SARS2 Spike glycoprotein
UniProt number P0DTC2
Protein class Single span transmembrane protein
Organism Severe acute respiratory syndrome coronavirus 2 (2019-nCoV) (SARS-CoV-2)
Mutation that differ from "original" SPIKE protein S13I
W152C
L452R
D1183Y
Further modifications
  • furin cleavage site “RRAR” mutated to “GSAG”; KV986PP; V987P
  • C-terminal Rho1D4 tag fused with spacer “GSSG” to protein sequence
Affinity tag C-terminal Rho1D4
Expression Host Hek293 Expi cells
Size 1286 amino acids (including Rho1D4 tag and linker)
142115.37 Da
Buffer composition 20 mM Hepes pH 7.5; 150 mM NaCl, 0.001 % LMNG
Function host cell surface receptor binding; fusion of virus membrane with host endosome membrane
Purity (SDS-PAGE) >98% as determined by SDS-PAGE, see Fig. 1 A and B
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