Pure & Active membrane proteins are challenging to obtain and keeping. Therefore we offer a selection of pure & active membrane proteins for your convience. MC4R (Melanocortin receptor 4) from
H. sapiens was expressend in SF9 (baculovirus) and purified with our high-quality
Rho1d4-Agarose
Fig. 1: Western Blot and SDS-PAGE of MC4R
Fig. 2: MC4R was purified via rho1d4 affinity chromatography
Features
| Protein | MC4R (Melanocortin receptor 4) |
| Alternative names | MC4-R, Melanocortin receptor 4 |
| UniProt number | P32245 |
| Protein class | GPCR (G-protein coupled receptor) |
| Organism | Human (Homo sapiens) |
| Sequence | Full-length, wildtype sequence GSSG spacer (grey) and C-terminal Rho1D4 tag (TETSQVAPA)(green)
MKTIIALSYIFCLVFA MVNSTHRGMH TSLHLWNRSS YRLHSNASES LGKGYSDGGC YEQLFVSPEV FVTLGVISLL ENILVIVAI AKNKNLHSPM YFFICSLAVA DMLVSVSNGS ETIVITLLNS TDTDAQSFTV N IDNVIDSV ICSSLLASIC SLLSIAVDRY FTIFYALQYH NIMTVKRVGI IISCIWAACT VS GILFIIY SDSSAVIICL ITMFFTMLAL MASLYVHMFL MARLHIKRIA VLPGTGAIRQ GAN MKGAIT LTILIGVFVV CWAPFFLHLI FYISCPQNPY CVCFMSHFNL YLILIMCNSI IDPL IYALR SQELRKTFKE IICCYPLGGL CDLSSRYENL YFQG GS SGTETSQVAPA
|
| Affinity tags | C-terminal Rho1D4 tag) |
| Size (excluding additional elements) | 39 kDa |
| Expression system | T. Ni insect cells (baculovirus expression system) |
| Purified via | Rho1D4 agarose |
| Buffer | 20 mM Hepes, pH 7.5; 100 mM NaCl; 2 mM EDTA; 0,1 % DM |
| Purity (SDS-PAGE) | >95 % as determined by SDS-PAGE |
| Homogeneity | Size exclusion chromatography |
| Function | Receptor specific to the heptapeptide core common to adrenocorticotropic hormone and alpha-, beta-, and gamma-MSH. Plays a central role in energy homeostasis and somatic growth. This receptor is mediated by G proteins that stimulate adenylate cyclase (cAMP) |
