NTSR1

NTSR1, a human GPCR binding the neurotransmitter neurotensin, is our latest addition to our portfolio of purified GPCRs, which also includes GLP1 receptor 1, G-protein coupled bile receptor 1 (TGR5), and mu-opioid receptor 1, reconstituted in nanodisc.

The protein is available off-the shelf, ready for ligand binding studies, crystallization, and other biochemical/biophysical experiments.

Protein	NTSR1 (Neurotensin receptor type 1)
Alternative names	High-affinity levocabastine-insensitive neurotensin receptor NTRH
UniProt number	P30989
Protein class	GPCR
Organism	Human (Homo sapiens)
Sequence	Full-length, wildtype sequence N-terminal HA tag (underlined), 6x His-tag (red), spacer (gray), Rho1D4 tag (green) MKTIIALSYIFCLVFAHHHHHHRLNSSAPGT PGTPAADPFQ RAQAGLEEAL LAPGFGNASG NASERVLAAP SSELDVNTDI YSKVLVTAVY LALFVVGTVG NTVTAFTLAR KKSLQSLQST VHYHLGSLAL SDLLTLLLAM PVELYNFIWV HHPWAFGDAG CRGYYFLRDA CTYATALNVA SLSVERYLAI CHPFKAKTLM SRSRTKKFIS AIWLASALLA VPMLFTMGEQ NRSADGQHAG GLVCTPTIHT ATVKVVIQVN TFMSFIFPMV VISVLNTIIA NKLTVMVRQA AEQGQVCTVG GEHSTFSMAI EPGRVQALRH GVRVLRAVVI AFVVCWLPYH VRRLMFCYIS DEQWTPFLYD FYHYFYMVTN ALFYVSSTIN PILYNLVSAN FRHIFLATLA CLCPVWRRRR KRPAFSRKAD SVSSNHTLSS NATRETLYGSSGTETSQVAPA
Affinity tags	N-terminal His / C-terminal Rho1D4
Size (excluding additional elements)	436 amino acids, 48,13 kDa
Expression system	Sf9 (baculovirus)
Purified via	Rho1D4 affinity chromatography, size exclusion chromatography
Buffer	50 mM HEPES, pH 8.0; 200 mM NaCl; 10% Glycerol; 0.1% n-Decyl-beta-maltoside; 0.01% cholesterolhemisuccinate; 0.01% CHAPSO
Purity (SDS-PAGE)	>95% as determined by SDS-PAGE
Homogeneity	Size exclusion chromatography
Activity	LNot determined
Function	Receptor for neurotensin. Involved in hypotension, hyperglycemia, hypothermia, antinociception, and regulation of intestinal motility and secretion.