The protein is available off-the shelf, ready for ligand binding studies, crystallization, and other biochemical/biophysical experiments.
Features
Protein | NTSR1 (Neurotensin receptor type 1) |
Alternative names | High-affinity levocabastine-insensitive neurotensin receptor NTRH |
UniProt number | P30989 |
Protein class | GPCR |
Organism | Human (Homo sapiens) |
Sequence | Full-length, wildtype sequence N-terminal HA tag (underlined), 6x His-tag (red), spacer (gray), Rho1D4 tag (green) MKTIIALSYIFCLVFAHHHHHHRLNSSAPGT PGTPAADPFQ RAQAGLEEAL LAPGFGNASG NASERVLAAP SSELDVNTDI YSKVLVTAVY LALFVVGTVG NTVTAFTLAR KKSLQSLQST VHYHLGSLAL SDLLTLLLAM PVELYNFIWV HHPWAFGDAG CRGYYFLRDA CTYATALNVA SLSVERYLAI CHPFKAKTLM SRSRTKKFIS AIWLASALLA VPMLFTMGEQ NRSADGQHAG GLVCTPTIHT ATVKVVIQVN TFMSFIFPMV VISVLNTIIA NKLTVMVRQA AEQGQVCTVG GEHSTFSMAI EPGRVQALRH GVRVLRAVVI AFVVCWLPYH VRRLMFCYIS DEQWTPFLYD FYHYFYMVTN ALFYVSSTIN PILYNLVSAN FRHIFLATLA CLCPVWRRRR KRPAFSRKAD SVSSNHTLSS NATRETLYGSSGTETSQVAPA |
Affinity tags | N-terminal His / C-terminal Rho1D4 |
Size (excluding additional elements) | 436 amino acids, 48,13 kDa |
Expression system | SF9 (baculovirus) |
Purified via | Rho1D4 affinity chromatography, size exclusion chromatography |
Buffer | 50 mM HEPES, pH 8.0; 200 mM NaCl; 10% Glycerol; 0.1% n-Decyl-beta-maltoside; 0.01% cholesterolhemisuccinate; 0.01% CHAPSO |
Purity (SDS-PAGE) | >95% as determined by SDS-PAGE |
Homogeneity | Size exclusion chromatography |
Activity | Not determined |
Function | Receptor for neurotensin. Involved in hypotension, hyperglycemia, hypothermia, antinociception, and regulation of intestinal motility and secretion. |