Sensory rhodopsin-1 (SR-1) is a 7-transmembrane protein that acts as a phototaxis receptor in archaebacteria. Due to its covalently bound colored ligand, SR-1 is ideal for use as a control membrane protein, for example for crystallization assays and biochemical/biophysical experiments. We provide SR-1 of high purity, purified from E.coli as a His-tagged protein.
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Figure 1: SDS-PAGE of sensory rhodopsin-1 from H. salinarum.
Figure 2: Absorbance spectrum of sensory rhodopsin-1 from H. salinarum.
|Protein ||Sensory rhodopsin-1 (SR-1, HsSRI) |
|UniProt number ||P0DMH8 |
|Protein class ||7-transmembrane protein |
|Organism ||Halobacterium salinarum S9 |
|Sequence || |
wild-type sequence 1-234 aa (lacking 15 aa at the C-terminus), 10x His-tag (red)
MDAVATAYLG GAVALIVGVA FVWLLYRSLD GSPHQSALAP LAIIPVFAGL SYVGMAYDIG TVIVNGNQIV GLRYIDWLVT TPILVGYVGY AAGASRRSII GVMVADALMI AVGAGAVVTD GTLKWALFGV SSIFHLSLFA YLYVIFPRVV PDVPEQIGLF NLLKNHIGLL WLAYPLVWLF GPAGIGEATA AGVALTYVFL DVLAKVPYVY FFYARRRVFM HSESHHHHHH HHHH
|Affinity tags ||His-tag (C-terminus) |
|Size ||234 amino acids; 25,423 Da |
|Expression system ||E.coli |
|Purified via ||PureCube Ni-NTA Agarose |
|Buffer ||4 M NaCl, 50 mM MES pH 6.0, 0.03% dodecyl maltoside (DDM) |
|Purity (SDS-PAGE) ||>98%, see Fig. 1 |
|Absorbance ||Extinction coefficient at 560 nm: 63,000 M-1 cm-1. |
|Activity ||Binding of ligand all-trans retinal, covalently bound to a lysine residue. Evaluation of UV-VIS spectrum: absorbance ratio 280 nm / 590 nm = 1.7 |
|Function ||Photoreceptor protein undergoing a photocycle, Retinal protein, phototaxis receptor |
|Literature references || |
- Radu, Ionela, et al. "Signal relay from sensory rhodopsin I to the cognate transducer HtrI: Assessing the critical change in hydrogen-bonding between Tyr-210 and Asn-53." Biophysical chemistry 150.1-3 (2010): 23-28.
- Schmies, Georg, Igor Chizhov, and Martin Engelhard. "Functional expression of His‐tagged sensory rhodopsin I in Escherichia coli." FEBS letters 466.1 (2000): 67-69.