Sensory Rhodopsin-1 (Hs)

Order number: 28911

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Description

Sensory rhodopsin-1 (SR-1) is a 7-transmembrane protein that acts as a phototaxis receptor in archaebacteria. Due to its covalently bound colored ligand, SR-1 is ideal for use as a control membrane protein, for example for crystallization assays and biochemical/biophysical experiments. We provide SR-1 of high purity, purified from E.coli as a His-tagged protein.
Feature
Alternative names Sensory rhodopsin-1 (SR-1, HsSRI)
UniProt Number P0DMH8
Protein class 7-transmembrane protein
Organism Halobacterium salinarum S9
Sequence, explained wild-type sequence 1-234 aa (lacking 15 aa at the C-terminus), 10x His-tag
Sequence, One-Letter Code MDAVATAYLG GAVALIVGVA FVWLLYRSLD GSPHQSALAP LAIIPVFAGL SYVGMAYDIG TVIVNGNQIV GLRYIDWLVT TPILVGYVGY AAGASRRSII GVMVADALMI AVGAGAVVTD GTLKWALFGV SSIFHLSLFA YLYVIFPRVV PDVPEQIGLF NLLKNHIGLL WLAYPLVWLF GPAGIGEATA AGVALTYVFL DVLAKVPYVY FFYARRRVFM HSESHHHHHH HHHH
Affinity tags His-tag (C-terminus)
Expression Host E.coli
Size (excluding additional elements) 234 amino acids; 25,423 Da
Buffer composition 4 M NaCl, 50 mM MES pH 6.0, 0.03% dodecyl maltoside (DDM)
Purity >98%, determined via SDS-PAGE (Figure 1)
Purified via PureCube Ni-NTA Agarose
Absorbance Extinction coefficient at 560 nm: 63,000 M-1 cm-1.
Activity Binding of ligand all-trans retinal, covalently bound to a lysine residue. Evaluation of UV-VIS spectrum: absorbance ratio 280 nm / 590 nm = 1.7
Function Photoreceptor protein undergoing a photocycle, Retinal protein, phototaxis receptor
Literature references
  1. Radu, Ionela, et al. "Signal relay from sensory rhodopsin I to the cognate transducer HtrI: Assessing the critical change in hydrogen-bonding between Tyr-210 and Asn-53." Biophysical chemistry 150.1-3 (2010): 23-28.
  2. Schmies, Georg, Igor Chizhov, and Martin Engelhard. "Functional expression of His‐tagged sensory rhodopsin I in Escherichia coli." FEBS letters 466.1 (2000): 67-69.

Lab Results

Sensory Rhodopsin-1  westernblot
Figure 1: SDS-PAGE of sensory rhodopsin-1 from H. salinarum..
Sensory Rhodopsin-1 absoprtion spectrum measured by Cube Biotech
Figure 2: Absorbance spectrum of sensory rhodopsin-1 from H. salinarum.

FAQ

Can I get the datasheet for the Sensory-Rhodopsin-1 protein?

Do you also offer other full-length membrane proteins?

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