Active, purified Sensory rhodopsin-1 (Halobacterium salinarum)
Sensory rhodopsin-1 (SR-1) is a 7-transmembrane protein that acts as a phototaxis receptor in archaebacteria. Due to its covalently bound colored ligand, SR-1 is ideal for use as a control membrane protein, for example for crystallization assays and biochemical/biophysical experiments. We provide SR-1 of high purity, purified from E.coli as a his-tagged protein.
Figure 1: SDS-PAGE of sensory rhodopsin-1 from H. salinarum.
Figure 2: Absorbance spectrum of sensory rhodopsin-1 from H. salinarum.
Sensory rhodopsin-1 (SR-1, HsSRI)
Halobacterium salinarum S9
wild-type sequence 1-234 aa (lacking 15 aa at the C-terminus), 10x His-tag (red)
MDAVATAYLGGAVALIVGVAFVWLLYRSLDGSPHQSALAPLAIIPVFAGLSYVGMAYDIGTV IVNGNQIVGLRYIDWLVTTPILVGYVGYAAGASRRSIIGVMVADALMIAVGAGAVVTDGTLK WALFGVSSIFHLSLFAYLYVIFPRVVPDVPEQIGLFNLLKNHIGLLWLAYPLVWLFGPAGIGEA TAAGVALTYVFLDVLAKVPYVYFFYARRRVFMHSESHHHHHHHHHH
234 amino acids; 25,423 Da
>98%, see Fig. 1
Extinction coefficient at 560 nm: 63,000 M-1 cm-1.
Extinction coefficient at 590 nm: 54,000 M-1 cm-1.
Binding of ligand all-trans retinal, covalently bound to a lysine residue. Evaluation of UV-VIS spectrum: absorbance ratio 280 nm / 590 nm = 1.7
Photoreceptor protein undergoing a photocycle, Retinal protein, phototaxis receptor
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