Sensory rhodopsin-1 (SR-1) is a 7-transmembrane protein that acts as a phototaxis receptor in archaebacteria. Due to its covalently bound colored ligand, SR-1 is ideal for use as a control membrane protein, for example for crystallization assays and biochemical/biophysical experiments. We provide SR-1 of high purity, purified from E.coli as a his-tagged protein.
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Figure 1: SDS-PAGE of sensory rhodopsin-1 from H. salinarum.
Figure 2: Absorbance spectrum of sensory rhodopsin-1 from H. salinarum.
Features
| Protein | Sensory rhodopsin-1 (SR-1, HsSRI) |
| UniProt number | P0DMH8 |
| Protein class | 7-transmembrane protein |
| Organism | Halobacterium salinarum S9 |
| Sequence | wild-type sequence 1-234 aa (lacking 15 aa at the C-terminus), 10x His-tag (red)
MDAVATAYLG GAVALIVGVA FVWLLYRSLD GSPHQSALAP LAIIPVFAGL SYVGMAYDIG TVIVNGNQIV GLRYIDWLVT TPILVGYVGY AAGASRRSII GVMVADALMI AVGAGAVVTD GTLKWALFGV SSIFHLSLFA YLYVIFPRVV PDVPEQIGLF NLLKNHIGLL WLAYPLVWLF GPAGIGEATA AGVALTYVFL DVLAKVPYVY FFYARRRVFM HSESHHHHHH HHHH
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| Affinity tags | His-tag (C-terminus) |
| Size | 234 amino acids; 25,423 Da |
| Expression system | E.coli |
| Purified via | PureCube Ni-NTA Agarose |
| Buffer | 4 M NaCl, 50 mM MES pH 6.0, 0.03% dodecyl maltoside (DDM) |
| Purity (SDS-PAGE) | >98%, see Fig. 1 |
| Absorbance | Extinction coefficient at 560 nm: 63,000 M-1 cm-1. |
| Activity | Binding of ligand all-trans retinal, covalently bound to a lysine residue. Evaluation of UV-VIS spectrum: absorbance ratio 280 nm / 590 nm = 1.7 |
| Function | Photoreceptor protein undergoing a photocycle, Retinal protein, phototaxis receptor |
| Literature references | - Radu, Ionela, et al. "Signal relay from sensory rhodopsin I to the cognate transducer HtrI: Assessing the critical change in hydrogen-bonding between Tyr-210 and Asn-53." Biophysical chemistry 150.1-3 (2010): 23-28.
- Schmies, Georg, Igor Chizhov, and Martin Engelhard. "Functional expression of His‐tagged sensory rhodopsin I in Escherichia coli." FEBS letters 466.1 (2000): 67-69.
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