Sensory rhodopsin-1 (Hs)


Proteins
Sensory rhodopsin-1 (SR-1) is a 7-transmembrane protein that acts as a phototaxis receptor in archaebacteria. Due to its covalently bound colored ligand, SR-1 is ideal for use as a control membrane protein, for example for crystallization assays and biochemical/biophysical experiments. We provide SR-1 of high purity, purified from E.coli as a his-tagged protein.
Please contact us for bulk inquiries, academic projects or collaboration ideas.

HsR1_SDS-Page
Figure 1: SDS-PAGE of sensory rhodopsin-1 from H. salinarum.
HsR1_absorbance;spectrum
Figure 2: Absorbance spectrum of sensory rhodopsin-1 from H. salinarum.

 

Features

Protein Sensory rhodopsin-1 (SR-1, HsSRI)
UniProt number P0DMH8
Protein class 7-transmembrane protein
Organism Halobacterium salinarum S9
Sequence

wild-type sequence 1-234 aa (lacking 15 aa at the C-terminus), 10x His-tag (red)


MDAVATAYLG GAVALIVGVA FVWLLYRSLD GSPHQSALAP LAIIPVFAGL SYVGMAYDIG TVIVNGNQIV GLRYIDWLVT TPILVGYVGY AAGASRRSII GVMVADALMI AVGAGAVVTD GTLKWALFGV SSIFHLSLFA YLYVIFPRVV PDVPEQIGLF NLLKNHIGLL WLAYPLVWLF GPAGIGEATA AGVALTYVFL DVLAKVPYVY FFYARRRVFM HSESHHHHHH HHHH

Affinity tags His-tag (C-terminus)
Size 234 amino acids; 25,423 Da
Expression system E.coli
Purified via PureCube Ni-NTA Agarose
Buffer 4 M NaCl, 50 mM MES pH 6.0, 0.03% dodecyl maltoside (DDM)
Purity (SDS-PAGE) >98%, see Fig. 1
Absorbance Extinction coefficient at 560 nm: 63,000 M-1 cm-1.
Activity Binding of ligand all-trans retinal, covalently bound to a lysine residue. Evaluation of UV-VIS spectrum: absorbance ratio 280 nm / 590 nm = 1.7
Function Photoreceptor protein undergoing a photocycle, Retinal protein, phototaxis receptor
Literature references
  1. Radu, Ionela, et al. "Signal relay from sensory rhodopsin I to the cognate transducer HtrI: Assessing the critical change in hydrogen-bonding between Tyr-210 and Asn-53." Biophysical chemistry 150.1-3 (2010): 23-28.
  2. Schmies, Georg, Igor Chizhov, and Martin Engelhard. "Functional expression of His‐tagged sensory rhodopsin I in Escherichia coli." FEBS letters 466.1 (2000): 67-69.
Proteins
Sensory rhodopsin-1 (SR-1) is a 7-transmembrane protein that acts as a phototaxis receptor in archaebacteria. Due to its covalently bound colored ligand, SR-1 is ideal for use as a control membrane protein, for example for crystallization assays and biochemical/biophysical experiments. We provide SR-1 of high purity, purified from E.coli as a his-tagged protein.
Please contact us for bulk inquiries, academic projects or collaboration ideas.

HsR1_SDS-Page
Figure 1: SDS-PAGE of sensory rhodopsin-1 from H. salinarum.
HsR1_absorbance;spectrum
Figure 2: Absorbance spectrum of sensory rhodopsin-1 from H. salinarum.

 

Features

Protein Sensory rhodopsin-1 (SR-1, HsSRI)
UniProt number P0DMH8
Protein class 7-transmembrane protein
Organism Halobacterium salinarum S9
Sequence

wild-type sequence 1-234 aa (lacking 15 aa at the C-terminus), 10x His-tag (red)


MDAVATAYLG GAVALIVGVA FVWLLYRSLD GSPHQSALAP LAIIPVFAGL SYVGMAYDIG TVIVNGNQIV GLRYIDWLVT TPILVGYVGY AAGASRRSII GVMVADALMI AVGAGAVVTD GTLKWALFGV SSIFHLSLFA YLYVIFPRVV PDVPEQIGLF NLLKNHIGLL WLAYPLVWLF GPAGIGEATA AGVALTYVFL DVLAKVPYVY FFYARRRVFM HSESHHHHHH HHHH

Affinity tags His-tag (C-terminus)
Size 234 amino acids; 25,423 Da
Expression system E.coli
Purified via PureCube Ni-NTA Agarose
Buffer 4 M NaCl, 50 mM MES pH 6.0, 0.03% dodecyl maltoside (DDM)
Purity (SDS-PAGE) >98%, see Fig. 1
Absorbance Extinction coefficient at 560 nm: 63,000 M-1 cm-1.
Activity Binding of ligand all-trans retinal, covalently bound to a lysine residue. Evaluation of UV-VIS spectrum: absorbance ratio 280 nm / 590 nm = 1.7
Function Photoreceptor protein undergoing a photocycle, Retinal protein, phototaxis receptor
Literature references
  1. Radu, Ionela, et al. "Signal relay from sensory rhodopsin I to the cognate transducer HtrI: Assessing the critical change in hydrogen-bonding between Tyr-210 and Asn-53." Biophysical chemistry 150.1-3 (2010): 23-28.
  2. Schmies, Georg, Igor Chizhov, and Martin Engelhard. "Functional expression of His‐tagged sensory rhodopsin I in Escherichia coli." FEBS letters 466.1 (2000): 67-69.
Top seller
Sensory rhodopsin-1 (Hs)
No results were found for the filter!
Sensory rhodopsin-1_Hs (100 ug) Sensory rhodopsin-1_Hs (100 ug)
Active, purified his-tagged membrane protein from Halobacterium salinarium. 100 microgram in solution. Shipped on dry ice - additional freight cost may apply.
Article number: 28911
Sales price: €300.00 *
Viewed