Sensory rhodopsin-2 (Hs)


Proteins
Sensory rhodopsin-2 (SR-2) is a 7-transmembrane protein that acts as a phototaxis receptor in archaebacteria. Due to its covalently bound colored ligand, SR-2 is ideal for use as a control membrane protein, for example for crystallization assays and biochemical/biophysical experiments. We provide SR-2 of high purity, purified from E. coli as a his-tagged protein.

Sensory_rhodpsin2_SDS-Page
Figure 1: SDS-PAGE of sensory rhodopsin-2 from H. salinarum.
Sensory_rhodpsin2_Absorbance
Figure 2: Absorbance spectrum of sensory rhodopsin-2 from H. salinarum.

 

Features

Protein Sensory rhodopsin-2 (SR-2, NpSRII)
UniProt number P71411
Protein class 7-transmembrane protein
Organism Halobacterium salinarum S9
Sequence

wild-type sequence, full-length, 6x His-tag (red)


MALTTWFWVG AVGMLAGTVL PIRDCIRHPS HRRYDLVLAG ITGLAAIAYT TMGLGITATT VGDRTVYLAR YIDWLVTTPL IVLYLAMLAR PGHRTSAWLL AADVFVIAAG IAAALTTGVQ RWLFFAVGAA GYAALLYGLL GTLPRALGDD PRVRSLFVTL RNITVVLWTL YPVVWLLSPA GIGILQTEMY TIVVVYLDFI SKVAFVAFAV LGADAVSRLV AADAAAPATA EPTPDGD HHHHHH

Affinity tags His-tag (C-terminus)
Size 243 amino acids; 26,103 Da
Expression system E.coli
Purified via PureCube Ni-NTA Agarose
Buffer 4 M NaCl, 50 mM MES pH 6.0, 0.03% dodecyl maltoside (DDM)
Purity (SDS-PAGE) >98%, see Fig. 1
Absorbance Extinction coefficient at 497 nm: 48,000 M-1 cm-1.
Absorbance Extinction coefficient at 590 nm: 54,000 M-1 cm-1.
Activity Binding of ligand all-trans retinal, covalently bound to a lysine residue. Evaluation of UV-VIS spectrum: absorbance ratio 280 nm / 497 nm = 1.7(additional vibronic bands at 460 nm and 420 nm)
Function Photoreceptor protein undergoing a photocycle, Retinal protein, photophobic phototaxis receptor
Literature references
  1. Mironova, O. S., et al. "Functional characterization of sensory rhodopsin II from Halobacterium salinarum expressed in Escherichia coli." FEBS letters 579.14 (2005): 3147-3151.
Proteins
Sensory rhodopsin-2 (SR-2) is a 7-transmembrane protein that acts as a phototaxis receptor in archaebacteria. Due to its covalently bound colored ligand, SR-2 is ideal for use as a control membrane protein, for example for crystallization assays and biochemical/biophysical experiments. We provide SR-2 of high purity, purified from E. coli as a his-tagged protein.

Sensory_rhodpsin2_SDS-Page
Figure 1: SDS-PAGE of sensory rhodopsin-2 from H. salinarum.
Sensory_rhodpsin2_Absorbance
Figure 2: Absorbance spectrum of sensory rhodopsin-2 from H. salinarum.

 

Features

Protein Sensory rhodopsin-2 (SR-2, NpSRII)
UniProt number P71411
Protein class 7-transmembrane protein
Organism Halobacterium salinarum S9
Sequence

wild-type sequence, full-length, 6x His-tag (red)


MALTTWFWVG AVGMLAGTVL PIRDCIRHPS HRRYDLVLAG ITGLAAIAYT TMGLGITATT VGDRTVYLAR YIDWLVTTPL IVLYLAMLAR PGHRTSAWLL AADVFVIAAG IAAALTTGVQ RWLFFAVGAA GYAALLYGLL GTLPRALGDD PRVRSLFVTL RNITVVLWTL YPVVWLLSPA GIGILQTEMY TIVVVYLDFI SKVAFVAFAV LGADAVSRLV AADAAAPATA EPTPDGD HHHHHH

Affinity tags His-tag (C-terminus)
Size 243 amino acids; 26,103 Da
Expression system E.coli
Purified via PureCube Ni-NTA Agarose
Buffer 4 M NaCl, 50 mM MES pH 6.0, 0.03% dodecyl maltoside (DDM)
Purity (SDS-PAGE) >98%, see Fig. 1
Absorbance Extinction coefficient at 497 nm: 48,000 M-1 cm-1.
Absorbance Extinction coefficient at 590 nm: 54,000 M-1 cm-1.
Activity Binding of ligand all-trans retinal, covalently bound to a lysine residue. Evaluation of UV-VIS spectrum: absorbance ratio 280 nm / 497 nm = 1.7(additional vibronic bands at 460 nm and 420 nm)
Function Photoreceptor protein undergoing a photocycle, Retinal protein, photophobic phototaxis receptor
Literature references
  1. Mironova, O. S., et al. "Functional characterization of sensory rhodopsin II from Halobacterium salinarum expressed in Escherichia coli." FEBS letters 579.14 (2005): 3147-3151.
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Sensory rhodopsin-2 (Hs)
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Sensory rhodopsin-2_Hs (100 ug) Sensory rhodopsin-2_Hs (100 ug)
Active, purified his-tagged membrane protein from Halobacterium salinarium. 100 microgram in solution. Shipped on dry ice - additional freight cost may apply.
Article number: 28921
Sales price: €300.00 *
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