Sensory Rhodopsin-2 (Hs)

Order number: 28921

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Description

Sensory rhodopsin-2 (SR-2) is a 7-transmembrane helice protein that acts as a phototaxis receptor in archaebacteria. Due to its covalently bound colored ligand, SR-2 is ideal for use as a control membrane protein, for example for crystallization assays and biochemical/biophysical experiments. We provide SR-2 of high purity, purified from E.coli as a His-tagged protein.
Feature
Alternative names Sensory rhodopsin-2 (SR-2, NpSRII)
UniProt Number P71411
Protein class 7-transmembrane protein
Organism Halobacterium salinarum S9
Sequence, explained wild-type sequence, full-length, 6x His-tag
Sequence, One-Letter Code MALTTWFWVG AVGMLAGTVL PIRDCIRHPS HRRYDLVLAG ITGLAAIAYT TMGLGITATT VGDRTVYLAR YIDWLVTTPL IVLYLAMLAR PGHRTSAWLL AADVFVIAAG IAAALTTGVQ RWLFFAVGAA GYAALLYGLL GTLPRALGDD PRVRSLFVTL RNITVVLWTL YPVVWLLSPA GIGILQTEMY TIVVVYLDFI SKVAFVAFAV LGADAVSRLV AADAAAPATA EPTPDGD HHHHHH
Affinity tags His-tag (C-terminus)
Expression Host E.coli
Size 243 amino acids; 26,103 Da
Buffer composition 4 M NaCl, 50 mM MES pH 6.0, 0.03% dodecyl maltoside (DDM)
Purity >98%, determined via SDS-PAGE (Figure 1)
Purified via PureCube Ni-NTA Agarose
Absorbance Extinction coefficient at 497 nm: 48,000 M-1 cm-1..
Extinction coefficient at 590 nm: 54,000 M-1 cm-1.
Activity Binding of ligand all-trans retinal, covalently bound to a lysine residue. Evaluation of UV-VIS spectrum: absorbance ratio 280 nm / 497 nm = 1.7(additional vibronic bands at 460 nm and 420 nm)
Function Photoreceptor protein undergoing a photocycle, Retinal protein, photophobic phototaxis receptor
Literature references
  1. Mironova, O. S., et al. "Functional characterization of sensory rhodopsin II from Halobacterium salinarum expressed in Escherichia coli." FEBS letters 579.14 (2005): 3147-3151.

Lab Results

Sensory Rhodopsin-1  westernblot
Figure 1: SDS-PAGE of sensory rhodopsin-2 from N. pharaonis.
Sensory Rhodopsin-1 absoprtion spectrum measured by Cube Biotech
Figure 2: Absorbance spectrum of sensory rhodopsin-2 from N. pharaonis.

FAQ

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