Sensory rhodopsin-2 (SR-2) is a 7-transmembrane protein that acts as a phototaxis receptor in archaebacteria. Due to its covalently bound colored ligand, SR-2 is ideal for use as a control membrane protein, for example for crystallization assays and biochemical/biophysical experiments. We provide SR-2 of high purity, purified from E. coli as a his-tagged protein.
Figure 1: SDS-PAGE of sensory rhodopsin-2 from N. pharaonis.
Figure 2: Absorbance spectrum of sensory rhodopsin-2 from N. pharaonis.
|Protein ||Sensory rhodopsin-2 (SR-2, NpSRII) |
|UniProt number ||P42196 |
|Protein class ||7-transmembrane protein |
|Organism ||Natronomonas pharaonis (Natronobacterium pharaonis) |
|Sequence || |
wild-type sequence, full-length, 7x His-tag (red)
MVGLTTLFWL GAIGMLVGTL AFAWAGRDAG SGERRYYVTL VGISGIAAVA YVVMALGVGW VPVAERTVFA PRYIDWILTT PLIVYFLGLL AGLDSREFGI VITLNTVVML AGFAGAMVPG IERYALFGMG AVAFLGLVYY LVGPMTESAS QRSSGIKSLY VRLRNLTVIL WAIYPFIWLL GPPGVALLTP TVDVALIVYL DLVTKVGFGF IALDAAATLR AEHGESLAGV DTDAPAVADH HHHHHH
|Affinity tags ||His-tag (C-terminus) |
|Size ||246 amino acids; 26,315 Da |
|Expression system ||E.coli |
|Purified via ||PureCube Ni-NTA Agarose |
|Buffer ||4 M NaCl, 300 mM NaCl, 50 mM MES pH 6.0, 0.03% dodecyl maltoside (DDM) |
|Purity (SDS-PAGE) ||>98%, see Fig. 1 |
|Absorbance ||Extinction coefficient at 500 nm: 45,500 M-1 cm-1. |
|Absorbance ||Extinction coefficient at 590 nm: 54,000 M-1 cm-1. |
|Activity ||Binding of ligand all-trans retinal, covalently bound to a lysine residue. Evaluation of UV-VIS spectrum: absorbance ratio 280 nm / 500 nm = 2.8 |
|Function ||Photoreceptor protein undergoing a photocycle, Retinal protein, photophobic phototaxis receptor |
|Literature references || |
- Gordeliy, Valentin I., et al. "Molecular basis of transmembrane signalling by sensory rhodopsin II–transducer complex." Nature 419.6906 (2002): 484.
- Orekhov, Philipp, et al. "Sensory rhodopsin I and sensory rhodopsin II form trimers of dimers in complex with their cognate transducers." Photochemistry and photobiology 93.3 (2017): 796-804.