Sensory Rhodopsin-2 (Np)

Order number: 28931

€411.00*

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Description

Sensory rhodopsin-2 (SR-2) from N. pharaonis. is a 7-transmembrane protein that acts as a phototaxis receptor in archaebacteria. Due to its covalently bound colored ligand, SR-2 is ideal for use as a control membrane protein, for example for crystallization assays and biochemical/biophysical experiments. We provide SR-2 of high purity, purified from E.coli  as a His-tagged protein.
Feature
Alternative names Sensory rhodopsin-2 (SR-2, NpSRII)
UniProt Number P42196
Protein class 7-transmembrane protein
Organism Natronomonas pharaonis (Natronobacterium pharaonis)
Sequence, explained wild-type sequence, full-length, 7x His-tag
Sequence, One-Letter Code MVGLTTLFWL GAIGMLVGTL AFAWAGRDAG SGERRYYVTL VGISGIAAVA YVVMALGVGW VPVAERTVFA PRYIDWILTT PLIVYFLGLL AGLDSREFGI VITLNTVVML AGFAGAMVPG IERYALFGMG AVAFLGLVYY LVGPMTESAS QRSSGIKSLY VRLRNLTVIL WAIYPFIWLL GPPGVALLTP TVDVALIVYL DLVTKVGFGF IALDAAATLR AEHGESLAGV DTDAPAVADH HHHHHH
Affinity tags His-tag (C-terminus)
Expression Host E.coli
Size 246 amino acids; 26,315 Da
Buffer composition 4 M NaCl, 300 mM NaCl, 50 mM MES pH 6.0, 0.03% dodecyl maltoside (DDM)
Purity >98%, determined via SDS-PAGE (Figure 1)
Purified via PureCube Ni-NTA Agarose
Absorbance Extinction coefficient at 500 nm: 45,500 M-1 cm-1..
Extinction coefficient at 590 nm: 54,000 M-1 cm-1.
Activity Binding of ligand all-trans retinal, covalently bound to a lysine residue. Evaluation of UV-VIS spectrum: absorbance ratio 280 nm / 500 nm = 2.8
Function Photoreceptor protein undergoing a photocycle, Retinal protein, photophobic phototaxis receptor
Literature references
  1. Gordeliy, Valentin I., et al. "Molecular basis of transmembrane signalling by sensory rhodopsin II–transducer complex." Nature 419.6906 (2002): 484.
  2. Orekhov, Philipp, et al. "Sensory rhodopsin I and sensory rhodopsin II form trimers of dimers in complex with their cognate transducers." Photochemistry and photobiology 93.3 (2017): 796-804.

Lab Results

Sensory Rhodopsin-1  westernblot
Figure 1: SDS-PAGE of sensory rhodopsin-2 from N. pharaonis.
Sensory Rhodopsin-1 absoprtion spectrum measured by Cube Biotech
Figure 2: Absorbance spectrum of sensory rhodopsin-2 from N. pharaonis.

FAQ

Can I get the datasheet for the Sensory-Rhodopsin-2 (Np) protein?

Do you also offer other full-length membrane proteins?

Yes, we do. Just browse through our product protofolio. In case we do not have your desired membrane protein in our store, send a request to our protein services.