Sensory rhodopsin-2 (Np)


Proteins
Sensory rhodopsin-2 (SR-2) is a 7-transmembrane protein that acts as a phototaxis receptor in archaebacteria. Due to its covalently bound colored ligand, SR-2 is ideal for use as a control membrane protein, for example for crystallization assays and biochemical/biophysical experiments. We provide SR-2 of high purity, purified from E. coli as a his-tagged protein.

SDS-Page; Sensory Rhodopsin2
Figure 1: SDS-PAGE of sensory rhodopsin-2 from N. pharaonis.
Absorbance Sensory Rhodopsin2
Figure 2: Absorbance spectrum of sensory rhodopsin-2 from N. pharaonis.

 

Features

Protein Sensory rhodopsin-2 (SR-2, NpSRII)
UniProt number P42196
Protein class 7-transmembrane protein
Organism Natronomonas pharaonis (Natronobacterium pharaonis)
Sequence

wild-type sequence, full-length, 7x His-tag (red)


MVGLTTLFWL GAIGMLVGTL AFAWAGRDAG SGERRYYVTL VGISGIAAVA YVVMALGVGW VPVAERTVFA PRYIDWILTT PLIVYFLGLL AGLDSREFGI VITLNTVVML AGFAGAMVPG IERYALFGMG AVAFLGLVYY LVGPMTESAS QRSSGIKSLY VRLRNLTVIL WAIYPFIWLL GPPGVALLTP TVDVALIVYL DLVTKVGFGF IALDAAATLR AEHGESLAGV DTDAPAVADH HHHHHH

Affinity tags His-tag (C-terminus)
Size 246 amino acids; 26,315 Da
Expression system E.coli
Purified via PureCube Ni-NTA Agarose
Buffer 4 M NaCl, 300 mM NaCl, 50 mM MES pH 6.0, 0.03% dodecyl maltoside (DDM)
Purity (SDS-PAGE) >98%, see Fig. 1
Absorbance Extinction coefficient at 500 nm: 45,500 M-1 cm-1.
Absorbance Extinction coefficient at 590 nm: 54,000 M-1 cm-1.
Activity Binding of ligand all-trans retinal, covalently bound to a lysine residue. Evaluation of UV-VIS spectrum: absorbance ratio 280 nm / 500 nm = 2.8
Function Photoreceptor protein undergoing a photocycle, Retinal protein, photophobic phototaxis receptor
Literature references
  1. Gordeliy, Valentin I., et al. "Molecular basis of transmembrane signalling by sensory rhodopsin II–transducer complex." Nature 419.6906 (2002): 484.
  2. Orekhov, Philipp, et al. "Sensory rhodopsin I and sensory rhodopsin II form trimers of dimers in complex with their cognate transducers." Photochemistry and photobiology 93.3 (2017): 796-804.
Proteins
Sensory rhodopsin-2 (SR-2) is a 7-transmembrane protein that acts as a phototaxis receptor in archaebacteria. Due to its covalently bound colored ligand, SR-2 is ideal for use as a control membrane protein, for example for crystallization assays and biochemical/biophysical experiments. We provide SR-2 of high purity, purified from E. coli as a his-tagged protein.

SDS-Page; Sensory Rhodopsin2
Figure 1: SDS-PAGE of sensory rhodopsin-2 from N. pharaonis.
Absorbance Sensory Rhodopsin2
Figure 2: Absorbance spectrum of sensory rhodopsin-2 from N. pharaonis.

 

Features

Protein Sensory rhodopsin-2 (SR-2, NpSRII)
UniProt number P42196
Protein class 7-transmembrane protein
Organism Natronomonas pharaonis (Natronobacterium pharaonis)
Sequence

wild-type sequence, full-length, 7x His-tag (red)


MVGLTTLFWL GAIGMLVGTL AFAWAGRDAG SGERRYYVTL VGISGIAAVA YVVMALGVGW VPVAERTVFA PRYIDWILTT PLIVYFLGLL AGLDSREFGI VITLNTVVML AGFAGAMVPG IERYALFGMG AVAFLGLVYY LVGPMTESAS QRSSGIKSLY VRLRNLTVIL WAIYPFIWLL GPPGVALLTP TVDVALIVYL DLVTKVGFGF IALDAAATLR AEHGESLAGV DTDAPAVADH HHHHHH

Affinity tags His-tag (C-terminus)
Size 246 amino acids; 26,315 Da
Expression system E.coli
Purified via PureCube Ni-NTA Agarose
Buffer 4 M NaCl, 300 mM NaCl, 50 mM MES pH 6.0, 0.03% dodecyl maltoside (DDM)
Purity (SDS-PAGE) >98%, see Fig. 1
Absorbance Extinction coefficient at 500 nm: 45,500 M-1 cm-1.
Absorbance Extinction coefficient at 590 nm: 54,000 M-1 cm-1.
Activity Binding of ligand all-trans retinal, covalently bound to a lysine residue. Evaluation of UV-VIS spectrum: absorbance ratio 280 nm / 500 nm = 2.8
Function Photoreceptor protein undergoing a photocycle, Retinal protein, photophobic phototaxis receptor
Literature references
  1. Gordeliy, Valentin I., et al. "Molecular basis of transmembrane signalling by sensory rhodopsin II–transducer complex." Nature 419.6906 (2002): 484.
  2. Orekhov, Philipp, et al. "Sensory rhodopsin I and sensory rhodopsin II form trimers of dimers in complex with their cognate transducers." Photochemistry and photobiology 93.3 (2017): 796-804.
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Sensory rhodopsin-2 (Np)
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Sensory rhodopsin-2 (N. pharaonis ,100 µg) Sensory rhodopsin-2 (N. pharaonis ,100 µg)
Sensory rhodopsin-2 from Natronomonas pharaonis. Ideal for use as a control membrane protein. Ready to order and use. 100 µg
Article number: 28931
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