PureCube Affinity Purification Matrices
Once expressed proteins are extracted from cell lysates or membranes, affinity chromatography is an elegant way to purify these to homogeneity in very few steps. We use BioWorks Workbeads as the basis to synthesize PureCube affinity purification matrices. This ensures the optimal quality we demand for our service projects. Our product offering includes agarose resins for batch and column-based purification as well as PureCube MagBeads
for purification using a magnet. The latter are especially useful when purifying from dilute solutions, such as cell culture supernatants, or for pull-down experiments. To detect recombinant proteins in a Western Blot, Cube Biotech offers tag-specific antibodies
The His tag
is the most widely used affinity tag because of its small size (6-10 aa) and ease of use. His-tag affinity purification can be performed under native and denaturing conditions and tolerates a wide range of detergents and other additives. A range of different His affinity resins is available, with different ligands and bead sizes. Refer to our overview page
for more information.
The GST tag is a 26 kDa protein tag which is often chosen to promote recombinant protein folding.
is a 8 amino acid tag with high specificity for StrepTactin® matrices which is often used as alternative or in combination with the His tag.
is a 9 amino acid tag that binds to the rho1D4 antibody and has proven particularly efficient in the purification of membrane proteins. For the first time commercially available, Cube Biotech offers the full system (affinity matrix, rho1D4 peptide, and rho1D4 antibody).
Please note that Ion exchange matrices are no longer available.
can be used to couple your own antibody or other molecule to create a protein-specific affinity matrix. Available modifications include
Special and Custom Matrices can be used for tag-free purification, purification of native proteins and specific protein conformations. Learn more
StrepTactin® and Strep-tag® are trademarks of IBA GmbH.