PureCube Affinity Purification Matrices | Cube Biotech

Purification Resins


PureCube Affinity Purification Matrices

Once expressed proteins are extracted from cell lysates or membranes, affinity chromatography is an elegant way to purify these to homogeneity in very few steps. We use BioWorks Workbeads as the basis to synthesize PureCube affinity purification matrices. This ensures the optimal quality we demand for our service projects. Our product offering includes agarose resins for batch and column-based purification as well as PureCube MagBeads for purification using a magnet. The latter are especially useful when purifying from dilute solutions, such as cell culture supernatants, or for pull-down experiments. To detect recombinant proteins in a Western Blot, Cube Biotech offers tag-specific antibodies.
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Agaroses

The His tag is the most widely used affinity tag because of its small size (6-10 aa) and ease of use. His-tag affinity purification can be performed under native and denaturing conditions and tolerates a wide range of detergents and other additives. A range of different His affinity resins is available, with different ligands and bead sizes. Refer to our overview page for more information.

The GST tag is a 26 kDa protein tag which is often chosen to promote recombinant protein folding.

The Strep-tag® is a 8 amino acid tag with high specificity for StrepTactin® matrices which is often used as alternative or in combination with the His tag.

The rho1D4-tag is a 9 amino acid tag that binds to the rho1D4 antibody and has proven particularly efficient in the purification of membrane proteins. For the first time commercially available, Cube Biotech offers the full system (affinity matrix, rho1D4 peptide, and rho1D4 antibody).

Please note that Ion exchange matrices are no longer available.

Untagged proteins can also be purified via hydrophobic interaction matrices. Especially suited as a second step after ion exchange chromatography, different HIC materials such as Butyl, Octyl, and Phenyl Agarose are available.

Extra-large matrices can be used for automated purification, and for other special applications.

Activated Matrices can be used to couple your own antibody or other molecule to create a protein-specific affinity matrix. Available modifications include

Special and Custom Matrices can be used for tag-free purification, purification of native proteins and specific protein conformations. Learn more

StrepTactin® and Strep-tag® are trademarks of IBA GmbH.
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Purification Resins

PureCube Affinity Purification Matrices

Once expressed proteins are extracted from cell lysates or membranes, affinity chromatography is an elegant way to purify these to homogeneity in very few steps. We use BioWorks Workbeads as the basis to synthesize PureCube affinity purification matrices. This ensures the optimal quality we demand for our service projects. Our product offering includes agarose resins for batch and column-based purification as well as PureCube MagBeads for purification using a magnet. The latter are especially useful when purifying from dilute solutions, such as cell culture supernatants, or for pull-down experiments. To detect recombinant proteins in a Western Blot, Cube Biotech offers tag-specific antibodies.
Logo_Crystallization
 
Agaroses

The His tag is the most widely used affinity tag because of its small size (6-10 aa) and ease of use. His-tag affinity purification can be performed under native and denaturing conditions and tolerates a wide range of detergents and other additives. A range of different His affinity resins is available, with different ligands and bead sizes. Refer to our overview page for more information.

The GST tag is a 26 kDa protein tag which is often chosen to promote recombinant protein folding.

The Strep-tag® is a 8 amino acid tag with high specificity for StrepTactin® matrices which is often used as alternative or in combination with the His tag.

The rho1D4-tag is a 9 amino acid tag that binds to the rho1D4 antibody and has proven particularly efficient in the purification of membrane proteins. For the first time commercially available, Cube Biotech offers the full system (affinity matrix, rho1D4 peptide, and rho1D4 antibody).

Please note that Ion exchange matrices are no longer available.

Untagged proteins can also be purified via hydrophobic interaction matrices. Especially suited as a second step after ion exchange chromatography, different HIC materials such as Butyl, Octyl, and Phenyl Agarose are available.

Extra-large matrices can be used for automated purification, and for other special applications.

Activated Matrices can be used to couple your own antibody or other molecule to create a protein-specific affinity matrix. Available modifications include

Special and Custom Matrices can be used for tag-free purification, purification of native proteins and specific protein conformations. Learn more

StrepTactin® and Strep-tag® are trademarks of IBA GmbH.
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Purification Resins
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Rho Starter Set 1: PureCube Rho1D4 Agarose (1 ml) + Rho1D4 peptide (5 mg) Rho Starter Set 1: PureCube Rho1D4 Agarose (1...
Set of 2 ml 50% Rho1D4 Agarose and 5 mg lyophilized Rho1D4 peptide. Provides a 50% discount compared to purchase of individual items.
Article number: 33199
Sales price: €167.00 *
PureCube Ni-NTA Agarose PureCube Ni-NTA Agarose
2 ml 50% NTA Agarose, loaded with nickel (II) sulfate
Article number: 31101
Sales price: From €12.00 *
PureCube 100 Ni-NTA Agarose PureCube 100 Ni-NTA Agarose
2 ml 50% NTA Agarose, with 100 micron average bead size. Loaded with nickel (II) sulfate
Article number: 74103
Sales price: From €105.00 *
PureCube 100 INDIGO Ni-Agarose PureCube 100 INDIGO Ni-Agarose
50% Agarose of 100 micron average bead size, equipped with a proprietary ligand for His affinity purification. Loaded with nickel (II) sulfate. For more information read:...
Article number: 75103
Sales price: From €105.00 *
PureCube Glutathione Agarose PureCube Glutathione Agarose
2 ml 50% Glutathione Agarose
Article number: 32101
Sales price: From €19.00 *
PureCube Rho1D4 Agarose PureCube Rho1D4 Agarose
2 ml 50% Rho1D4 Agarose
Article number: 33101
Sales price: From €227.00 *
PureCube NHS Activated Agarose PureCube NHS Activated Agarose
2 ml 50% NHS-Activated Agarose, for direct coupling of biomolecules via amine groups For more information read here: https://cube-biotech.com/products/purification-resins/activated-resins/nhs-activated-agarose/#
Article number: 50303
Sales price: From €112.00 *
PureCube Ni-IDA Agarose PureCube Ni-IDA Agarose
2 ml 50% IDA Agarose, loaded with nickel (II) sulfate
Article number: 30103
Sales price: From €68.00 *
PureCube IDA Agarose PureCube IDA Agarose
2 ml 50% IDA Agarose - for loading with transition metals
Article number: 30703
Sales price: From €68.00 *
PureCube NTA Agarose PureCube NTA Agarose
2 ml 50% NTA Agarose - for loading with transition metals
Article number: 31701
Sales price: From €12.00 *
PureCube Amine Activated Agarose PureCube Amine Activated Agarose
2 ml 50% Amine-Activated Agarose, for coupling of biomolecules via carboxy groups using EDC/NHS For more information read here: https://cube-biotech.com/products/purification-resins/activated-resins/amine-activated-agarose/#
Article number: 51003
Sales price: From €100.00 *
PureCube Maleimide Activated Agarose PureCube Maleimide Activated Agarose
2 ml 50% Maleimide-Activated Agarose, for direct, irreversible coupling of biomolecules via thiol groups For more information read here:...
Article number: 51103
Sales price: From €295.00 *
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