PureCube Affinity Purification Matrices | Cube Biotech

Purification Resins


Purification Resins
Once expressed proteins are extracted from cell lysates or membranes, affinity chromatography is an elegant way to purify these to homogeneity in very few steps. We use BioWorks Workbeads as the basis to synthesize PureCube affinity purification matrices. This ensures the optimal quality we demand for our service projects. Our product offering includes agarose resins for batch and column-based purification as well as PureCube MagBeads for purification using a magnet. The latter are especially useful when purifying from dilute solutions, such as cell culture supernatants, or for pull-down experiments. To detect recombinant proteins in a Western Blot, Cube Biotech offers.
 

Table 1: Different tags for different applications
TagSpecificationProtein bindingProducts
His Due to its very small size it rarely affects protein function his tagged proteins His-Affinity Resins
GST The GST tag is a 26 kDa protein tag which is often chosen to promote recombinant protein folding. GST tagged proteins GST Affinity Resins
Strep The Strep-tag® is a 8 amino acid tag with high specificity for StrepTactin® magbeads which is often used as alternative or in combination with the His tag Strep tagged proteins Strep Affinity Resins
rho1d4 The rho1D4-tag is a 9 amino acid tag that binds to the rho1D4 antibody and has proven particularly efficient in the purification of membrane proteins. For the first time commercially available, Cube Biotech offers the full system (affinity magbeads, rho1D4 peptide, and rho1D4 antibody). rho1d4 tagged proteins Rho1D4 Affinity Resins
Carboxy Resin carry a free carboxyl group Amine Carboxy Agarose
NHS Resin carry a N-hydroxy-succinimide (NHS) functionality Amine NHS Activated Agarose
Amine Resin carry a free amino group Carboxy Amine Activated Agarose
Epoxy Resin carry a free epoxy group Amine or Thiol Epoxy Activated Agarose
Maleimide Resin carry a free maleimide group thiol Maleimide Activated Agarose
Purification Resins
Once expressed proteins are extracted from cell lysates or membranes, affinity chromatography is an elegant way to purify these to homogeneity in very few steps. We use BioWorks Workbeads as the basis to synthesize PureCube affinity purification matrices. This ensures the optimal quality we demand for our service projects. Our product offering includes agarose resins for batch and column-based purification as well as PureCube MagBeads for purification using a magnet. The latter are especially useful when purifying from dilute solutions, such as cell culture supernatants, or for pull-down experiments. To detect recombinant proteins in a Western Blot, Cube Biotech offers.
 

Table 1: Different tags for different applications
TagSpecificationProtein bindingProducts
His Due to its very small size it rarely affects protein function his tagged proteins His-Affinity Resins
GST The GST tag is a 26 kDa protein tag which is often chosen to promote recombinant protein folding. GST tagged proteins GST Affinity Resins
Strep The Strep-tag® is a 8 amino acid tag with high specificity for StrepTactin® magbeads which is often used as alternative or in combination with the His tag Strep tagged proteins Strep Affinity Resins
rho1d4 The rho1D4-tag is a 9 amino acid tag that binds to the rho1D4 antibody and has proven particularly efficient in the purification of membrane proteins. For the first time commercially available, Cube Biotech offers the full system (affinity magbeads, rho1D4 peptide, and rho1D4 antibody). rho1d4 tagged proteins Rho1D4 Affinity Resins
Carboxy Resin carry a free carboxyl group Amine Carboxy Agarose
NHS Resin carry a N-hydroxy-succinimide (NHS) functionality Amine NHS Activated Agarose
Amine Resin carry a free amino group Carboxy Amine Activated Agarose
Epoxy Resin carry a free epoxy group Amine or Thiol Epoxy Activated Agarose
Maleimide Resin carry a free maleimide group thiol Maleimide Activated Agarose
Purification Resins
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PureCube Ni-NTA Agarose PureCube Ni-NTA Agarose
2 ml 50% NTA Agarose, loaded with nickel (II) sulfate
Article number: 31101
Sales price: From €12.00 *
PureCube NHS Activated Agarose XL PureCube NHS Activated Agarose XL
20 ml 50% NHS-Activated Agarose, for direct coupling of biomolecules via amine groups. Medium particle size of 400 micrometer, for special applications
Article number: 58303
Sales price: From €140.00 *
PureCube 100 INDIGO Ni-Agarose PureCube 100 INDIGO Ni-Agarose
50% Agarose of 100 micron average bead size, equipped with a proprietary ligand for His affinity purification. Loaded with nickel (II) sulfate. For more information read:...
Article number: 75103
Sales price: From €105.00 *
Rho Starter Set 1: PureCube Rho1D4 Agarose (1 ml) + Rho1D4 peptide (5 mg) Rho Starter Set 1: PureCube Rho1D4 Agarose (1...
Rho1D4 of 2 ml 50% Rho1D4 Agarose and 5 mg lyophilized Rho1D4 peptide. ► 50% discount compared to the individual products
Article number: 33199
Sales price: €167.00 *
PureCube Rho1D4 Agarose PureCube Rho1D4 Agarose
2 ml 50% Rho1D4 Agarose
Article number: 33101
Sales price: From €227.00 *
PureCube Ni-IDA Agarose PureCube Ni-IDA Agarose
2 ml 50% IDA Agarose, loaded with nickel (II) sulfate
Article number: 30103
Sales price: From €68.00 *
PureCube NTA Agarose PureCube NTA Agarose
2 ml 50% NTA Agarose - for loading with transition metals
Article number: 31701
Sales price: From €12.00 *
PureCube Maleimide Activated Agarose PureCube Maleimide Activated Agarose
2 ml 50% Maleimide-Activated Agarose, for direct, irreversible coupling of biomolecules via thiol groups For more information read here:...
Article number: 51103
Sales price: From €295.00 *
PureCube Fe-NTA Agarose PureCube Fe-NTA Agarose
Our PurceCube NTA-Agarose loaded with iron (III) chloride. Perfect for purification of phosphorylated proteins. 20ml
Article number: 31403-Fe
Sales price: From €104.00 *
PureCube DEAE Agarose PureCube DEAE Agarose
20 ml 50% DEAE Agarose, weak anion exchanger with diethylaminoethanol function
Article number: 38303
Sales price: From €25.00 *
PureCube HIC Starter Set (10 ml Butyl, Phenyl, Octyl Agarose each) PureCube HIC Starter Set (10 ml Butyl, Phenyl,...
Set consisting of 20 ml 50% Butyl Agarose, 20 ml 50% Phenyl Agarose, and 20 ml 50% Octyl Agarose. For screening of hydrophobic interaction conditions.
Article number: 39099
Sales price: €137.00 *
PureCube NHS Activated Agarose PureCube NHS Activated Agarose
2 ml 50% NHS-Activated Agarose, for direct coupling of biomolecules via amine groups For more information read here: https://cube-biotech.com/products/purification-resins/activated-resins/nhs-activated-agarose/#
Article number: 50303
Sales price: From €112.00 *
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