Once expressed proteins are extracted from cell lysates or membranes, affinity chromatography is an elegant way to purify these to homogeneity in very few steps. We use BioWorks Workbeads as the basis to synthesize PureCube affinity purification matrices. This ensures the optimal quality we demand for our service projects. Our product offering includes agarose resins for batch and column-based purification as well as PureCube MagBeads
for purification using a magnet. The latter are especially useful when purifying from dilute solutions, such as cell culture supernatants, or for pull-down experiments. To detect recombinant proteins in a Western Blot, Cube Biotech offers.
Table 1: Different tags for different applications
||Due to its very small size it rarely affects protein function
||his tagged proteins
||The GST tag is a 26 kDa protein tag which is often chosen to promote recombinant protein folding.
||GST tagged proteins
||GST Affinity Resins
||The Strep-tag® is a 8 amino acid tag with high specificity for StrepTactin® magbeads which is often used as alternative or in combination with the His tag
||Strep tagged proteins
||Strep Affinity Resins
||The rho1D4-tag is a 9 amino acid tag that binds to the rho1D4 antibody and has proven particularly efficient in the purification of membrane proteins. For the first time commercially available, Cube Biotech offers the full system (affinity magbeads, rho1D4 peptide, and rho1D4 antibody).
||rho1d4 tagged proteins
||Rho1D4 Affinity Resins
||Resin carry a free carboxyl group
||Resin carry a N-hydroxy-succinimide (NHS) functionality
||NHS Activated Agarose
||Resin carry a free amino group
||Amine Activated Agarose
||Resin carry a free epoxy group
||Amine or Thiol
||Epoxy Activated Agarose
||Resin carry a free maleimide group
||Maleimide Activated Agarose