PureCube Glutathione Agarose
The GST tag is a 26 kDa protein that binds to glutathione-coupled agarose resins. This tag is considerably larger than the His tag and is often chosen to promote recombinant protein folding. PureCube Glutathione Agarose is based on BioWorks Workbeads. For purification from cell culture supernatants or pull-down experiments, we recommend PureCube Glutathione MagBeads
. To detect GST-tagged proteins in Western Blot experiments, Cube Biotech offers a highly specific GST antibody.
PureCube Glutathione Agarose provides:
protein binding capacity of up to 10 mg/ mL
compatible with a range of detergents
ideal for purifying insoluble proteins
PureCube Glutathione Agarose was successfully used in the following publications:
For protein purification: van der Veen, A.G. et al. (2018) The RIG-I-like receptor LGPs inhibits Dicer-dependent processing of long double-stranded RNA and blocks RNA interference in mammalian cells. The EMBO Journal e97479
For pull-down experiments: Kilkenny, ML et al. (2017) The human CTF4-orthologue AND-1 interacts with DNA polymerase a/primase via its unique C-terminal HMG box. Open Biol. 7:170217.
Fig. 1: Purification yield is comparable to that of a market-leading resin. SDS-PAGE of GST expressed in E.coli and purified in gravity columns with PureCube Glutathione Agarose and the equivalent resin from Competitor G. Up to 10 mg/ml protein yield was obtained with PureCube Glutathione Agarose (elution fractions E1-E6, Cube)
High protein binding capacity
In a comparative study with equivalent, market-leading resins, PureCube Glutathione Agarose proved to be a competitive alternative, exhibiting the same protein binding capacity. Figure 1 is an SDS-PAGE of GST purified on gravity columns packed with PureCube Glutathione Agarose and the equivalent resin from Competitor G. Both resins yielded up to 10 mg/mL protein. TL: total lysate; CL: cleared lysate; FT: flow-through; W1/W4: wash fractions; E1-E6: elution fractions.