His Affinity Resins


Purification Resins
The polyhistidine tag is the most widely used affinity tag due to its small size, low immunogenicity, and versatility under native and denaturing conditions, as well as in presence of detergents and many other additives. Taking advantage of the affinity of transition metal ions for the imidazole ring of histidine, immobilized metal affinity chromatography (IMAC) is used to purify his-tagged proteins. Cube Biotech offers high-performance PureCube IDA Agarose and NTA Agarose, based on agarose with average 40 µm or 100 µm diameter. In addition, the novel INDIGO ligand provides optimal performance even in the presence of 20 mM EDTA and DTT.

PureCube His Affinity Agaroses provide:

Ready-to use His affinity agarose with 40 µm average bead size:
Ni-NTA Agarose Co-NTA Agarose Ni-IDA Agarose Other metals
Ready-to use His affinity agarose with 100 µm average bead size:
INDIGO Ni-Agarose 100 Ni-NTA Agarose 100 Co-NTA Agarose 100  
IMAC agaroses for loading with affinity metals:
NTA Agarose 100 NTA Agarose IDA Agarose  
 
Purification Resins
The polyhistidine tag is the most widely used affinity tag due to its small size, low immunogenicity, and versatility under native and denaturing conditions, as well as in presence of detergents and many other additives. Taking advantage of the affinity of transition metal ions for the imidazole ring of histidine, immobilized metal affinity chromatography (IMAC) is used to purify his-tagged proteins. Cube Biotech offers high-performance PureCube IDA Agarose and NTA Agarose, based on agarose with average 40 µm or 100 µm diameter. In addition, the novel INDIGO ligand provides optimal performance even in the presence of 20 mM EDTA and DTT.

PureCube His Affinity Agaroses provide:

Ready-to use His affinity agarose with 40 µm average bead size:
Ni-NTA Agarose Co-NTA Agarose Ni-IDA Agarose Other metals
Ready-to use His affinity agarose with 100 µm average bead size:
INDIGO Ni-Agarose 100 Ni-NTA Agarose 100 Co-NTA Agarose 100  
IMAC agaroses for loading with affinity metals:
NTA Agarose 100 NTA Agarose IDA Agarose  
 
Top seller
PureCube Ni-NTA Agarose PureCube Ni-NTA Agarose
Article number: 31103
PureCube Ni-IDA Agarose PureCube Ni-IDA Agarose
Article number: 30103
PureCube NTA Agarose PureCube NTA Agarose
Article number: 31701
PureCube 100 Ni-NTA Agarose PureCube 100 Ni-NTA Agarose
Article number: 74103
PureCube IDA Agarose PureCube IDA Agarose
Article number: 30703
PureCube Co-NTA Agarose PureCube Co-NTA Agarose
Article number: 31403
PureCube 100 Co-NTA Agarose PureCube 100 Co-NTA Agarose
Article number: 74403
His Affinity Resins
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PureCube 100 INDIGO Ni-Agarose PureCube 100 INDIGO Ni-Agarose
50% Agarose of 100 micron average bead size, equipped with a proprietary ligand for His affinity purification. Loaded with nickel (II) sulfate. For more information read:...
Article number: 75103
Sales price: From €84.00 * €105.00 *
PureCube Ni-NTA Agarose PureCube Ni-NTA Agarose
2 ml 50% NTA Agarose, loaded with nickel (II) sulfate
Article number: 31103
Sales price: From €78.40 * €98.00 *
PureCube Ni-IDA Agarose PureCube Ni-IDA Agarose
2 ml 50% IDA Agarose, loaded with nickel (II) sulfate
Article number: 30103
Sales price: From €54.40 * €68.00 *
PureCube NTA Agarose PureCube NTA Agarose
2 ml 50% NTA Agarose - for loading with transition metals
Article number: 31701
Sales price: From €12.00 *
PureCube 100 Ni-NTA Agarose PureCube 100 Ni-NTA Agarose
2 ml 50% NTA Agarose, with 100 micron average bead size. Loaded with nickel (II) sulfate
Article number: 74103
Sales price: From €84.00 * €105.00 *
PureCube IDA Agarose PureCube IDA Agarose
2 ml 50% IDA Agarose - for loading with transition metals
Article number: 30703
Sales price: From €68.00 *
PureCube Co-NTA Agarose PureCube Co-NTA Agarose
20 ml 50% NTA Agarose, loaded with cobalt (II) chloride
Article number: 31403
Sales price: From €83.20 * €104.00 *
PureCube 100 Co-NTA Agarose PureCube 100 Co-NTA Agarose
2 ml 50% NTA Agarose, with 100 micron average bead size. Loaded with cobalt (II) chloride
Article number: 74403
Sales price: From €88.80 * €111.00 *
PureCube 100 NTA Agarose PureCube 100 NTA Agarose
2 ml 50% NTA Agarose, with 100 micron average bead size. For loading with transition metals.
Article number: 74703
Sales price: From €105.00 *
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