PureCube His Affinity Agarose
The polyhistidine tag is the most widely used affinity tag due to its small size, low immunogenicity, and versatility under native and denaturing conditions, as well as in presence of detergents and many other additives. Taking advantage of the affinity of transition metal ions for the imidazole ring of histidine, immobilized metal affinity chromatography (IMAC) is used to purify his-tagged proteins. Cube Biotech offers high-performance PureCube IDA Agarose and NTA Agarose, based on agarose with average 40 µm or 100 µm diameter. In addition, the novel INDIGO ligand provides optimal performance even in the presence of 20 mM EDTA and DTT.
PureCube His Affinity Agaroses provide:
Ready-to use His affinity agarose with 40 µm average bead size
Ready-to use His affinity agarose with 100 µm average bead size
IMAC agaroses for loading with affinity metals