PureCube IDA Agarose
The His tag is the most widely used affinity tag due to its small size and versatility under native and denaturing conditions, as well as in presence of detergents and many other additives. Cube Biotech offers high-performance PureCube IDA Agarose, based on BioWorks Workbeads. IDA Agarose can be loaded with transition metals to obtain different specificities for his-tagged proteins, or for other applications such as purification of phosphorylated proteins. IDA Agarose is also available as pre-packed cartridge for FPLC applications. For purification from diluted solutions, IDA MagBeads
are recommended. For higher specificity, we offer the same high-quality resin with an NTA ligand. (what's the difference?
Why PureCube IDA Agarose?
Can be loaded with most transition metals
Stable under commonly used concentrations of DTT and EDTA
Can be regenerated for reuse